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Oomycetes form a deep lineage of eukaryotic organisms that includes a large number of plant pathogens which threaten natural and managed ecosystems. We undertook a survey to query the community for their ranking of plant-pathogenic oomycete species based on scientific and economic importance. In total, we received 263 votes from 62 scientists in 15(More)
A resistance gene analog (RGA)-derived sequence-characterized amplified region (SCAR) marker was successfully developed based on sequence homology with disease resistance genes of an AFLP molecular marker tightly linked to the Rl adg gene of Solanum tuberosum ssp. andigena. The new marker was designated as ‘RGASC850’ (RGA-derived SCAR) based on the size of(More)
A large number of quantitative trait loci (QTL) for resistance to late blight of potato have been reported with a “conventional” method in which each phenotypic trait reflects the cumulative genetic effects for the duration of the disease process. However, as genes controlling response to disease may have unique contributions with specific temporal(More)
Potato genotypes from a breeding population adapted to tropical highlands were analyzed for the stability of late blight resistance and also for marker-phenotype association. We harmonized the historical evaluation data, consisting of observations spanning 6 years from two field sites utilizing a resistance scale constructed by comparing the area under the(More)
Conserved ortholog set (COS) markers are an important functional genomics resource that has greatly improved orthology detection in Asterid species. A comprehensive list of these markers is available at Sol Genomics Network ( http://solgenomics.net/ ) and many of these have been placed on the genetic maps of a number of solanaceous species. We amplified(More)
Two Solanum genotypes, a wild relative of cultivated potato S. cajamarquense (Cjm) and an advanced tetraploid clone B3C1 (B3), were inoculated with two Phytophthora infestans isolates and leaves were sampled at 72 and 96 h after inoculation. Gene expression in the inoculated versus noninoculated samples was monitored using the Institute of Genomic Research(More)
The Rpi-blb2 gene (R gene) from the Mexican wild potato species, Solanum bulbocastanum, was introduced into the potato variety Desiree by agro-infection in order to confer resistance to late blight which is still the most important disease of the potato worldwide. A total of 148 putative transgenic events was obtained by selection based on hygromycin(More)
Tuber appearance is highly variable in the Andean cultivated potato germplasm. The diploid backcross mapping population ‘DMDD’ derived from the recently sequenced genome ‘DM’ represents a sample of the allelic variation for tuber shape and eye depth present in the Andean landraces. Here we evaluate the utility of morphological descriptors for tuber shape(More)
Ten potato genotypes with field resistance to bacterial wilt were evaluated for resistance to five strains of Ralstonia solanacearum belonging to biovars 1, 2A, 2T and 3 (phylotypes I and II) under greenhouse conditions. The plants were inoculated by pouring bacterial suspension into the soil and wilt incidence was recorded weekly. Stems and tubers of(More)
The aim of this study was to increase marker density in a previously identified late blight resistance QTL in a diploid potato population. SSR markers from the reference potato genome sequence were located by e-PCR in the QTL region in the chromosome 9 genetic map of the diploid potato population B3C1HP, and utilized to identify more candidate genes(More)