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Turkey erythrocyte membranes were solubilized in the mild detergent octylpenta(oxyethylene) [CH3(CH2)7-(OCH2CH2)5OH], which possesses a high critical micelle concentration (approximately equal to 6 mM) and forms small, dynamic micelles. Both the native enzyme Ns(GDP) X C and the p[NH]ppG-preactivated species N's X p[NH]ppG X C' were found to possess the(More)
Cells of the cyanobiont Anabaena azollae isolated from the water fern Azolla filiculoides were found to take up and utilize fructose in the light for mixotrophic growth. Fructose was favored by the cyanobiont as a substrate over sucrose and glucose. Cell growth in the presence of 8 mM fructose led to glycogen accumulation in the cells which approached 20%(More)
The guanylyl imidodiphosphate-activated state of turkey erythrocyte cyclase can be reversed to the basal state by the simultaneous action of beta-agonists and GTP. The rate of reversal diminishes progressively with decreasing concentration of beta-adrenergic receptors on the membrane surface, whereas the extent of reversal is always maximal. The rate of(More)
Mn2+ at concentrations below 0.1 mM supports the activation of turkey erythrocyte adenylate cyclase (ATP pyrophosphate-lyase (cyclizing), EC 4.6.1.1) by beta-agonists or NaF, similarly to Mg2+ at 5.0 mM. At higher concentrations, Mn2+ is strongly inhibitory, as is Mg2+ above 6 mM. Also, Mn2+ with GTP, but in the absence of beta-agonist, is very potent in(More)
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