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Switchable ion channels that are made of membrane proteins play different roles in cellular circuits. Since gating nanopore channels made of proteins can only work in the environment of lipid membrane, they are not fully compatible to the application requirement as a component of those nanodevice systems in which lipid membranes are hard to establish. Here(More)
A simple but highly specific immunoassay system for goat anti-human IgG has been developed using gold nanoparticles and microfluidic techniques. The assay is based on the deposition of gold nanoparticles that are coated with protein antigens in the presence of their corresponding antibodies to microfluidic channel surface. The effects of time accumulation,(More)
An electrochemical detection method was introduced for aqueous droplet analysis in oil phase of microfluidic devices. This method is based on the electrochemical signal difference between aqueous and oil. Applying a low alternating current (AC) voltage to a couple of Au microelectrodes, this method can offer size information and ion concentration range from(More)
We present a simple technique for cell loading, culturing, and phenotypic study in a multi-chamber microfluidic device made of polydimethylsiloxane (PDMS). This technique is based on the use of degassing induced aspiration of PDMS which allows loading cells into micro-cavities within 1 min. A large number of triangle cavities are patterned aside main flow(More)
A microfluidic device combined with the microwell array and optical tweezers was set up for cell manipulation, localization and cultivation. Yeast cells were manipulated by a 1,064 nm laser and transferred to microwell array as a demonstration. The flow velocities at which the yeast cell can be confined in microwells of different sizes are characterized.(More)
Recently, the experimentally observed asymmetric properties of ion transport in charged conical nanopores (CCNs) that resemble those in biological ion channels have attracted a lot of attention in theoretical studies in nanotechnology research. In this paper, we report several tactics to study this effect by directly solving the Poisson-Nernst-Planck (PNP)(More)
We present a simple and high-throughput microfluidic system for diffusion-based monolayer yeast cell culture monitoring. Yeast cells are patterned into the micro-cavity array with a suitable height (4 μm) that keeps the cells fixed in monolayer during the cell division. Different sizes of cavities and different repeating times of injection were tested in(More)
We have developed a microturbidostat for long time bacterial culture at constant density controlled by optical detection and integrated pneumatic valves. The device was fabricated by multilayer soft lithography and in-situ formation of an agarose filter. The culture chamber of bacteria was connected in one side to a single bacterial input-output channel and(More)