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—An indicated demand for sunfish (Lepomis spp.) as a food fish remains untested because of continuing inability to efficiently rear these fishes to required large sizes ($227 g; 0.5 lbs). A recent study involving parallel, indoor rearing of two sunfishes under favorable conditions showed that bluegills Lepomis macrochirus possessed markedly higher growth(More)
To perform whole genome scanning for complex trait analysis, we isolated and characterized a total of 21 novel genomic-SSRs and EST-SSRs for yellow perch (Perca flavescens), using the methods of construction of SSR-enrichment libraries and EST database mining of a related species P. fluviatilis. Of 16 genomic-SSR primer pairs examined, eight successfully(More)
Monosex populations are in demand in many fish species with sexual dimorphism, e.g., better growth performance, higher gonad value, superior ornamental value. From the point of view of research, a monosex population is one of the best materials for investigating sex-determining mechanisms, sex differentiation, and sex-linked markers. Sex reversal of females(More)
The molecular mechanisms that underlie sex determination and differentiation are conserved and diversified. In fish species, temperature-dependent sex determination and differentiation seem to be ubiquitous and molecular players involved in these mechanisms may be conserved. Although how the ambient temperature transduces signals to the undifferentiated(More)
As a prelude to strain selection for domestication and future marker assisted selection, genetic variation revealed by microsatellite DNA was evaluated in yellow perch, Perca flavescens, from four wild North American populations collected in 2003–2004 (Maine, New York, North Carolina, and Pennsylvania,), and two captive populations (Michigan and Ohio). For(More)
Yellow Perch Perca flavescens were subjected to handling stress and salt treatments at different temperatures to determine their physiological changes. Yellow Perch, held at 8–10◦C, were divided into three groups with four replicates and subjected to water temperatures of 14, 20, and 26◦C to acclimate. Then they were subjected to acute handling twice within(More)
Transcriptome sequencing could facilitate discovery of sex-biased genes, biological pathways and molecular markers, which could help clarify the molecular mechanism of sex determination and sexual dimorphism, and assist with selective breeding in aquaculture. Yellow perch has unique gonad system and sexual dimorphism and is an alternative model to study(More)
OBJECTIVE To determine the prevalence and genotypes of TEM- and SHV-type beta-lactamase-producing Klebsiella pneumoniae in Guangzhou area. METHODS Genotype determination of TEM- and SHV-type beta-lactamases were performed by PCR amplification followed by denaturing high-performance liquid chromatography (DHPLC) and DNA sequencing among 57 strain of(More)
There is increasing evidence that temperature effects on sex ratio in fish species are ubiquitous. Temperature effects on sex ratio could be influenced by parent, strain, and population, whether in fish species with temperature-dependent sex determination or genetic sex determination plus temperature effects. In the present study, effects of(More)