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Six new 4-phenyl-3,4-dihydroquinolone derivatives (1-6) along with the related aflaquinolone A (7) were isolated and identified from the cultures of Aspergillus nidulans MA-143, an endophytic fungus obtained from the fresh leaves of the marine mangrove plant Rhizophora stylosa. Their structures including absolute configurations were determined by(More)
In order to achieve quick and nondestructive prediction of cucumber disease, a prediction model of greenhouse cucumber downy mildew has been established and it is based on analysis technology of laser-induced chlorophyll fluorescence spectrum. By assaying the spectrum curve of healthy leaves, leaves inoculated with bacteria for three days and six days and(More)
Xiuyan, Liaoning was an important locality of jade material of the Red Mountain Culture in the Neolithic Age in Chinese history where there are two types of raw material of nephrite jades, namely gravel nephrite jade (Hemo Yu in Chinese) and old nephrite jade (Lao Yu in Chinese). In the present paper, Laser Raman spectrum technique was applied to analyze(More)
Soluble expression of recombinant therapeutic proteins in Escherichia coli (E. coli) has been a challenging task in biopharmaceutical development. In this study, a novel self-cleavable tag Zbasic–intein has been constructed for the soluble expression and purification of a recombinant cytokine, human interleukin-15 (IL-15). We screened several solubilizing(More)
The present paper is based on chlorophyll fluorescence spectrum analysis. The wavelength 685 nm was determined as the primary characteristic point for the analysis of healthy or disease and insect damaged leaf by spectrum configuration. Dimensionality reduction of the spectrum was achieved by combining simple intercorrelation bands selection and principal(More)
The infection and degree of cucumber aphis pests was studied by analyzing chlorophyllfluorescence spectrum in greenhouse. Based on the configuration of the spectrum, characteristic points were established, in which the intensity of waveband F632 was the first characteristic point between healthy and aphis pests leaves. The second characteristic point was K(More)
It has been documented that the purification of inclusion bodies from Escherichia coli by size exclusion chromatography (SEC) may benefit subsequent refolding and recovery of recombinant proteins. However, loading volume and the high cost of the column limits its application in large-scale manufacturing of biopharmaceutical proteins. We report a novel(More)
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