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Glucuronoyl Esterase Screening and Characterization Assays Utilizing Commercially Available Benzyl Glucuronic Acid Ester
TLDR
The three-level experimental procedure not merely facilitates routine, fast and simple biochemical characterizations but it can also give rise to the discovery of different GEs through an extensive screening of heterologous Genomic and Metagenomic expression libraries.
Neural progenitors organize in small-world networks to promote cell proliferation
TLDR
The results demonstrate that immature cells in the developing brain organize in small-world networks that critically regulate neural progenitor proliferation, and underscore the critical role of intricate cell signaling during embryonic development.
Separation of galactoglucomannans, lignin and lignin-carbohydrate complexes from hot-water-extracted Norway spruce by cross-flow filtration and adsorption chromatography
A simple method to simultaneously recover polymeric carbohydrates, mainly galactoglucomannans (GGM), lignin, and lignin-carbohydrate complex (LCC) from hot-water-extracted Norway spruce wood is
A GH115 α-glucuronidase from Schizophyllum commune contributes to the synergistic enzymatic deconstruction of softwood glucuronoarabinoxylan
TLDR
The authors' GH115 α-glucuronidase is capable of finely tailoring the molecular structure of softwood GAX, and contributes to the almost complete saccharification of GAX in synergy with other exo- and endo-xylan-acting enzymes.
Synthesis and enzymatic hydrolysis of a diaryl benzyl ester model of a lignin-carbohydrate complex (LCC)
Abstract Specific degradation of the bonds between lignin and carbohydrates is an important step towards separating individual lignocellulosic biopolymers for sustainable production of materials and
Combining Substrate Specificity Analysis with Support Vector Classifiers Reveals Feruloyl Esterase as a Phylogenetically Informative Protein Group
TLDR
Good correlation with the FAEs substrate specificities that are defined via the phylogenetic analysis not only suggests that FAEs are phylogenetically informative proteins but it is also a considerable step towards improved FAEs functional prediction.
Enzyme assays for lignin–carbohydrate bond hydrolysis
To identify, produce, and use enzymes, analytical methods known as enzyme assays are employed. Enzyme assays are based on analysing the changes brought about on a substrate by an enzyme under defined
Novel Enzymes for the degradation of Lignin-Carbohydrate complexes
In pulp and paper production, the delignification process damages the wood polymers irreversibly. A milder process based on enzymatic treatment could maintain various polymer properties that could be
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