Hamilton Fraval

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The Drosophila RhoGEF Pebble (Pbl) is required for cytokinesis and migration of mesodermal cells. In a screen for genes that could suppress migration defects in pbl mutants we identified the phosphatidylinositol phosphate (PtdInsP) regulator pi5k59B. Genetic interaction tests with other PtdInsP regulators suggested that PtdIns(4,5)P2 levels are important(More)
We have studied the response of human cells in culture to cis platinum[II] diammine dichloride (cis Pt[II]) induced DNA damage. The survival data, measured as a function of cis Pt[II] dose were similar in a normal cell line (Human foetal lung) compared to a UV-sensitive, thymine dimer excision repair-deficient cell line (Xeroderma pigmentosum). However,(More)
Platinum was lost from the DNA of cis-diamminedichloroplatinum(II) [cis-Pt(II)]-treated exponentially growing Chinese hamster V79-379A cells with a half-life of 28 hr. By contrast, platinum was lost from the DNA of cells treated in stationary-phase culture with a half-life of 4 days. Cells treated in and allowed to remain as a stationary-phase culture(More)
Methyl glyoxal inhibits the growth of Escherichia coli in synchronous and asynchronous culture. The inhibition of growth is accompanied by immediate inhibition of protein synthesis and of the initiation of replication of DNA. When methyl glyoxal is added after initiation of a round of replication the elongation of new DNA chains is not inhibited. Cell(More)
The assembly and constriction of an actomyosin contractile ring in cytokinesis is dependent on the activation of Rho at the equatorial cortex by a complex, here termed the cytokinesis initiation complex, between a microtubule-associated kinesin-like protein (KLP), a member of the RacGAP family, and the RhoGEF Pebble. Recently, the activity of the mammalian(More)
The mechanism by which cis-Platinum(II)diamminedichloride, (cis-Pt(II)), inhibited DNA synthesis was investigated using synchronously growing Chinese hamster V79-379A cells. The progression of G1 phase treated cells into S phase was not inhibited. However the rate of DNA synthesis throughout the S phase was depressed in a dose dependent manner compared to a(More)
The Rho GTP exchange factor, Pebble (Pbl), long recognised as an essential activator of Rho during cytokinesis, also regulates mesoderm migration at gastrulation. Like other cell cycle components, pbl expression patterns broadly correlate with proliferative tissue. Surprisingly, in spite of its role in the early mesoderm, pbl is downregulated in the(More)
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