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An Iranian National Quince collection containing 40 quince genotypes, originating from six distinct geographic areas, was screened using 15 SSR markers developed originally for apple and pear genomes. Overall, 13 markers exhibited polymorphism, with an average of 5.36 putative alleles per locus and a mean PIC value of 0.76. An UPGMA analysis divided the(More)
A reliable and rapid pathogen detection protocol that utilizes loop-mediated isothermal amplification (LAMP) was developed for detection of Erwinia amylovora, the casual agent of fire blight. The six LAMP primers applied were derived from the highly conserved fragment of the chromosomally amsH gene. Despite the proposed LAMP as well as nested PCR presenting(More)
OBJECTIVES Motility plays a major role in pathogenicity of Helicobacter pylori, yet there is scarce data regarding its chemotactic behaviour. The present study was designed to investigate the chemotactic responses of local isolates of H. pylori towards various sugars, amino acids, as well as some other chemical substances. MATERIALS AND METHODS Chemotaxis(More)
Here, the intensity and ratio of superoxide anion (O2 •), hydrogen peroxide (H2O2) and hydroxyl anion (OH•-) formation along the in vitro shootlets of four pear (Pyrus communis L.) rootstocks (i.e., Pyrodwarf, OH × F40, OH × F69 and OH × F333) were scrutinized under E. amylovora inoculation, over 144 hpi. Furthermore, following identifying the most tolerant(More)
The use rank annihilation factor analysis (RAFA) for spectrophotometric studies of complex formation equilibria are proposed. One-step complex formation and two successive and mononuclear complex formation systems studied successfully by proposed methods. When the complex stability constant acts as an optimizing object, and simply combined with the pure(More)
Erwinia amylovora is a necrogenic bacterium, causing the fire blight disease on many rosaceous plants. Triggering oxidative burst by E. amylovora is a key response by which host plants try to restrain pathogen spread. Electron transport chain (ETC) of chloroplasts is known as an inducible source of reactive oxygen species generation in various stresses.(More)
Attempts were made to identify eight pathogenesis related (PR) genes (i.e., PR-1a, PR3-ch1, PR3-Ch2, PR3-Ch3, PR3-Ch4, PR3-Ch5, PR-5 and PR-8) from 27 genotypes of apple, quince and pear, which are induced in response to inoculation with the pathogen Erwinia amylovora, the causal agent of fire blight. Totally, 32 PR genes of different families were(More)
BACKGROUND Clarithromycin resistance in Helicbacter pylori has been found to be associated with point mutations in 23s rRNA gene leads to reduced affinity of the antibiotic to its ribosomal target or changing the site of methylation. The aim of this study was to determine the most important point mutations in 23s rRNA gene in H. pylori that are closely(More)
METHOD: Nationwide Inpatient Sample (2006–2011) was queried to identify bilateral breast reduction cases. Patients with rheumatoid arthritis, systemic lupus erythematosus, Sjogren's syndrome, Raynaud's syndrome, psoriatic arthritis, or scleroderma were identified. Demographic factors, co-morbidities, and postoperative complications were compared to patients(More)