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In the present study, an electrochemical DNA sensing strategy for highly sensitivity detection of DNA hybridization was reported based on a gold nanoparticles (AuNPs) / cysteamine (Cys) / poly glutamic acid (PGA) modified electrode. A glassy electrode was first modified with the polymer film by cyclic voltammetry (CV), and then AuNPs were immobilized on the(More)
The present study aimed to determine the mechanism of cation-selective secretion by multicellular salt glands. Using a hydroponic culture system, the secretion and accumulation of Na+ and K+ in Tamarix ramosissima and T. laxa under different salt stresses (NaCl, KCl and NaCl+KCl) were studied. Additionally, the effects of salt gland inhibitors(More)
The protein tyrosine kinase Syk couples the B-cell receptor (BCR) for antigen to multiple intracellular signaling pathways and also modulates cellular responses to inducers of oxidative stress in a receptor-independent fashion. In B cells, Syk is found in both the nuclear and cytoplasmic compartments but contains no recognizable nuclear localization or(More)
The Streptomyces phage φC31 integrase can efficiently target attB-bearing transgenes to endogenous pseudo attP sites within mammalian genomes. To better understand the activity of φC31 integrase in the bovine genome, DNA sequences of 44 integration events were analyzed, and 32 pseudo attP sites were identified. The majority of these sites share a sequence(More)
Members of the marine dinoflagellate genus Alexandrium are known to exude allelochemicals, unrelated to well-known neurotoxins (PSP-toxins, spirolides), with negative effects on other phytoplankton and marine grazers. Physico/chemical characterization of extracellular lytic compounds of A. tamarense, quantified by Rhodomonas salina bioassay, showed that the(More)
We quantify the extreme heating associated with resonant irradiation of individual gold nanorods by using a novel assay based on partitioning of lipophilic dyes between membrane phases. The temperature increase is sensitively dependent on the angle between the laser polarization and the orientation of the nanorod. A dramatic and irreversible decrease in the(More)
Considering chaotic synchronization, the TCP protocol is usually adopted to transport encrypted data on networks at present. A block encryption algorithm is proposed to digital speech codes in this paper. The method uses the UDP protocol to cipher text. It partially solves the problem of decryption for receiver when some data packages are lost during(More)
BACKGROUND Kaposi's sarcoma (KS)-associated herpesvirus (KSHV) is the etiologic agent of KS, the most common AIDS-related malignancy. The majority of KS tumor cells harbor latent KSHV virus but only a small percentage undergoes spontaneous lytic replication. Viral reactivation from latency is crucial for the pathogenesis and development of KS, but the(More)
In this work, we present an electrochemical DNA sensor based on silver nanoparticles/poly(trans-3-(3-pyridyl) acrylic acid) (PPAA)/multiwalled carbon nanotubes with carboxyl groups (MWCNTs-COOH) modified glassy carbon electrode (GCE). The polymer film was electropolymerized onto MWCNTs-COOH modified electrode by cyclic voltammetry (CV), and then silver(More)
Streptomyces phage φC31 integrase induces efficient site-specific recombination capable of integrating exogenous genes at pseudo attP sites in human, mouse, rat, rabbit, sheep, Drosophila, and bovine genomes. However, the φC31-mediated recombination between attB and the corresponding pseudo attP sites has not been investigated in Capra hircus. Here, we(More)