Haisen Sun

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High-speed countercurrent chromatography (HSCCC) was used to separate kelp polysaccharide. HSCCC was performed using an aqueous two-phase solvent system composed of PEG1000-K₂HPO₄-KH₂PO₄-H₂O (0.5:1.25:1.25:7.0, w/w) by eluting a lower aqueous phase at 2.0 mL/min at 600 rpm, yielding two separate fractions, KPS-1 and KPS-2. The KPS-2 fraction was further(More)
OBJECTIVE To explore the mechanism of cholesterol-degrading by Lactobacillus plantarum LpT1 and LpT2 in vitro. METHODS After Lactobacillus plantarum LpT1 and LpT2 being inoculated and cultured in the medium MRS, MRS +CH, MRS + CH + S and MRS + CH + N, the cholesterol content in supernatant, deposit and cells, and the total cholesterol content before and(More)
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