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A β-glucosidase gene (bgl) from Aspergillus oryzae GIF-10 was cloned, sequenced and expressed. Its full-length DNA sequence was 2,903 bp and included three introns. The full-length cDNA sequence contained an open reading frame of 2,586 nucleotides, encoding 862 amino acids with a potential secretion signal. The A. oryzae GIF-10 bgl was functionally(More)
OBJECTIVES To evaluate the role of bcl-2 antisense oligodeoxynucleotide in the gene treatment of human laryngocarcinoma. METHODS An antisense oligodeoxynucleotide complementary to the initiation coden and the next four codens of bcl-2 mRNA was synthesized with an automatic DNA synthesizer. It was used to treat cultured Hep-2 cells to observe its effect on(More)
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