Hagai Kirshner

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Super resolution microscopy such as STORM and (F)PALM is now a well known method for biological studies at the nanometer scale. However, conventional imaging schemes based on sparse activation of photo-switchable fluorescent probes have inherently slow temporal resolution which is a serious limitation when investigating live-cell dynamics. Here, we present(More)
Localization microscopy relies on computationally efficient Gaussian approximations of the point spread function for the calculation of fluorophore positions. Theoretical predictions show that under specific experimental conditions, localization accuracy is significantly improved when the localization is performed using a more realistic model. Here, we show(More)
The quality of super-resolution images obtained by single-molecule localization microscopy (SMLM) depends largely on the software used to detect and accurately localize point sources. In this work, we focus on the computational aspects of super-resolution microscopy and present a comprehensive evaluation of localization software packages. Our philosophy is(More)
Super-resolution localization microscopy methods such as PALM and STORM have been shown to provide imaging with resolutions up to a few tens of nanometers while using relatively simple setups. Biplane PALM has extended the PALM technique to three-dimensions, by simultaneously using two imaging planes, with different focal depths. A key aspect in achieving(More)
This paper is devoted to the characterization of an extended family of CARMA (continuous-time autoregressive moving average) processes that are solutions of stochastic differential equations driven by white Lévy innovations. These are completely specified by: (1) a set of poles and zeros that fixes their correlation structure, and (2) a canonical(More)
Super-resolution localization microscopy relies on sparse activation of photo-switchable probes. Such activation, however , introduces limited temporal resolution. High-density imaging overcomes this limitation by allowing several neighboring probes to be activated simultaneously. In this work, we propose an algorithm that incorporates a continuous-domain(More)
Many sources of information are of analog or continuous-time nature. However, digital signal processing applications rely on discrete data. We consider the problem of approximating L<sub>2</sub> inner products, i.e., representation coefficients of a continuous-time signal, from its generalized samples. Adopting a robust approach, we process these(More)
— This paper is devoted to the characterization of an extended family of continuous-time autoregressive moving average (CARMA) processes that are solutions of stochastic differential equations driven by white Lévy innovations. These are completely specified by: 1) a set of poles and zeros that fixes their correlation structure and 2) a canonical infinitely(More)