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The development of cancer is contingent on the emergence of at least one clone of transformed cells. One method used to investigate whether human tumours are monoclonal depends on the mosaicism in the normal tissues of women heterozygous for the two forms of the enzyme glucose-6-phosphate dehydrogenase (G-6-PD). This mosaicism results from the inactivation(More)
The mouse hematopoietic system was subjected to repeated depletion and regeneration either by serial transfer of bone marrow cells through lethally irradiated recipients or by repeated treatment with the cycle-active drug hydroxyurea (HU). The capacity of surviving stem cells to proliferate and self-renew was assayed at intervals by two methods: (a) the(More)
The xid (X-linked immunodeficiency) mutation in the CBA/N mouse strain has been used extensively in studies of the functional and lineage relationships of B lymphocyte subpopulations (1-7). Defects associated with CBA/N (xid/xid) mice include the inability to respond to some thymus-independent antigens, hyporesponsiveness to some B cell mitogens and(More)
Earlier studies have suggested that splenic T cell populations in nursling mice (less than 18 d of age) have Lyt cell surface antigens that identify them as less mature than their adult counterparts. Studies presented here, however, demonstrate that the expression of the Thy-1, Lyt-1, Lyt-2, and Lyt-3 T cell antigens is virtually identical in 14-d-old and(More)
The TATA of two clones from the same murine methylcholanthrene-induced fibrosarcoma have been investigated by immunizing syngeneic mice with irradiated cells of one or both clones and challenging them 14 days later with viable cells. The tumour had been induced in a female backcross CBA mouse heterozygous for the A and B alloenzymes of phosphoglycerate(More)
The changes in transmembrane electrical potential (TMP) of Concanavalin A (Con-A)-stimulated lymphocytes from young adult and aged CBA/Ca mice were studied with a potential-sensitive fluorescent oxonol probe. The initial effect of Con-A was to depolarise lymphocytes from young mice and abrogated in the presence of tetraethylammonium chloride (TEA), an(More)
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