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Evolutionary conservation among biotin enzymes.
PURIFICATION AND PROPERTIES OF ENZYMES INVOLVED IN THE PROPIONIC ACID FERMENTATION
- S. Allen, R. Kellermeyer, R. Stjernholm, H. Wood
- Biology, Medicine
- Journal of bacteriology
- 1 January 1964
Methods for the separation and purification of phosphotransacetylase, acetyl kinase, malic dehydrogenase and coenzyme A (CoA) transferase, and the role of these enzymes in the propionic acid fermentation as well as the possible mechanism responsible for the high yields of adenosine triphosphate from glucose are considered. Expand
Isolation and characterization of a pyrophosphate-dependent phosphofructokinase from Propionibacterium shermanii.
It is proposed that (a) PPi may replace ATP in the formation of fructose-1,6-P2 when the organism is grown on glucose and (b) when the organisms are grown on lactate or glycerol the conversion ofructose-1-6- P2 to fructose- 6-P during gluconeogenesis may occur by phosphorolysis rather than hydrolysis. Expand
Biological aspects of inorganic polyphosphates.
Purification of five components from Clostridium thermoaceticum which catalyze synthesis of acetate from pyruvate and methyltetrahydrofolate. Properties of phosphotransacetylase.
A five-component enzyme system which catalyzes synthesis of acetylphosphate from methyltetrahydrofolate plus pyruvate has been purified from the homoacetate-fermenting bacterium, Clostridium thermoaceticum, and some properties of the phosphotransacetylase are presented. Expand
Synthesis of Acetyl Coenzyme A from Carbon Monoxide, Methyltetrahydrofolate, and Coenzyme A by Enzymes from Clostridium thermoaceticum
It is proposed that these reactions are part of the mechanism which enables certain autotrophic bacteria to grow on CO and that CH3THF is synthesized from CO and tetrahydrofolate which then is converted to acetyl-CoA, which serves as a precursor in other anabolic reactions. Expand
The acetyl-CoA pathway of autotrophic growth
It is concluded that the formation of acetyl-CoA by other acetogens, the methanogens and sulphate-reducing bacteria occurs by a mechanism very similar to that of C. thermoaceticum. Expand
Preparation of standards and determination of sizes of long-chain polyphosphates by gel electrophoresis.
This electrophoresis procedure allows, for the first time, the determination of the range of sizes present and the average chain length with only 2-20 micrograms of polyphosphate. Expand