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Single-Stranded Antisense siRNAs Guide Target RNA Cleavage in RNAi
Small interfering RNAs (siRNAs) are the mediators of mRNA degradation in the process of RNA interference (RNAi). Here, we describe a human biochemical system that recapitulates siRNA-mediated targetExpand
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Molecular Anatomy of a Trafficking Organelle
Membrane traffic in eukaryotic cells involves transport of vesicles that bud from a donor compartment and fuse with an acceptor compartment. Common principles of budding and fusion have emerged, andExpand
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Identification of Novel Argonaute-Associated Proteins
RNA silencing processes are guided by small RNAs known as siRNAs and microRNAs (miRNAs) . They reside in ribonucleoprotein complexes, which guide the cleavage of complementary mRNAs or affectExpand
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EF-P Is Essential for Rapid Synthesis of Proteins Containing Consecutive Proline Residues
Translating Polyproline Translation of messenger RNA into protein is carried out by the ribosome, together with a variety of accessory factors, which offer the potential for regulation of thisExpand
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RNA targeting by the type III-A CRISPR-Cas Csm complex of Thermus thermophilus.
CRISPR-Cas is a prokaryotic adaptive immune system that provides sequence-specific defense against foreign nucleic acids. Here we report the structure and function of the effector complex of the TypeExpand
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Characterization of novel SF3b and 17S U2 snRNP proteins, including a human Prp5p homologue and an SF3b DEAD‐box protein
Mass spectrometry was used to identify novel proteins associated with the human 17S U2 snRNP and one of its stable subunits, SF3b. Several additional proteins were identified, demonstrating that 17SExpand
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Small Nuclear Ribonucleoprotein Remodeling During Catalytic Activation of the Spliceosome
Major structural changes occur in the spliceosome during its activation just before catalyzing the splicing of pre–messenger RNAs (pre-mRNAs). Whereas changes in small nuclear RNA (snRNA)Expand
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GraFix: sample preparation for single-particle electron cryomicroscopy
We developed a method, named GraFix, that considerably improves sample quality for structure determination by single-particle electron cryomicroscopy (cryo-EM). GraFix uses a glycerol gradientExpand
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Proteomic and functional analysis of Argonaute‐containing mRNA–protein complexes in human cells
Members of the Argonaute (Ago) protein family associate with small RNAs and have important roles in RNA silencing. Here, we analysed Ago1‐ and Ago2‐containing protein complexes in human cells.Expand
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The evolutionarily conserved core design of the catalytic activation step of the yeast spliceosome.
Metazoan spliceosomes exhibit an elaborate protein composition required for canonical and alternative splicing. Thus, the minimal set of proteins essential for activation and catalysis remainsExpand
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