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Semi‐rational engineering of a coral fluorescent protein into an efficient highlighter
TLDR
The molecular cloning and crystal structure determination of a new fluorescent protein, KikG, from the coral Favia favus, and its in vitro evolution conferring green‐to‐red photoconvertibility are reported, leading to the generation of an efficient highlighter, KikGR.
Improving membrane voltage measurements using FRET with new fluorescent proteins
TLDR
A voltage sensor is developed that displays ∼40% changes in emission ratio per 100 mV, allowing for direct visualization of electrical activities in cultured excitable cells and has fast on-off kinetics at warm temperatures.
mKikGR, a Monomeric Photoswitchable Fluorescent Protein
TLDR
Molecule cloning and spectroscopic characterization of mKikGR, a monomeric version of the previously reported KikGR that displays high photostability and switching rates are reported, suggesting their suitability for super-resolution imaging.
Improved detection of electrical activity with a voltage probe based on a voltage‐sensing phosphatase
TLDR
An improved voltage probe named Mermaid2 is developed that provides ratiometric readouts of electrical activity with fast kinetics and great sensitivity, and was able to detect single‐event electrical activity both in vitro and in vivo.
GnRH-immunoreactive neuronal system in the presumptive ancestral chordate, Ciona intestinalis (Ascidian).
TLDR
The morphology of the GnRH neuronal system of ascidian, Ciona intestinalis, is examined by immunocytochemistry and many GnRH-immunoreactive neuronal cells and fibers are found in a specific surface area of the cerebral ganglion, along the inner wall of the dorsal blood sinus, as well as on the anterior surface of the ovary.
Strategies for Sperm Chemotaxis in the Siphonophores and Ascidians: A Numerical Simulation Study
TLDR
Two models of chemotaxis, one in the siphonophores and the second in the ascidians, are proposed based on experimental data and the properties of these strategies and future problems are discussed in relation to these models.
Functional diversity of voltage‐sensing phosphatases in two urodele amphibians
TLDR
Both VSPs showed “sensing” currents, indicating that their voltage sensor domains are functional, and the phosphatase activity of Cp‐VSP was found to be voltage dependent, as shown by its ability to regulate the conductance of coexpressed GIRK2 channels, but Hn‐V SP lacked such phosphat enzyme activity due to the truncation of its C2 domain.
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