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An auxin-based degron system for the rapid depletion of proteins in nonplant cells
Plants have evolved a unique system in which the plant hormone auxin directly induces rapid degradation of the AUX/IAA family of transcription repressors by a specific form of the SCF E3 ubiquitinExpand
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A novel ring-like complex of Xenopus proteins essential for the initiation of DNA replication.
We have identified Xenopus homologs of the budding yeast Sld5 and its three interacting proteins. These form a novel complex essential for the initiation of DNA replication in Xenopus egg extracts.Expand
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Localization of MCM2-7, Cdc45, and GINS to the site of DNA unwinding during eukaryotic DNA replication.
Little is known about the architecture and biochemical composition of the eukaryotic DNA replication fork. To study this problem, we used biotin-streptavidin-modified plasmids to induceExpand
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The N-Terminal Noncatalytic Region of Xenopus RecQ4 Is Required for Chromatin Binding of DNA Polymerase α in the Initiation of DNA Replication
ABSTRACT Recruitment of DNA polymerases onto replication origins is a crucial step in the assembly of eukaryotic replication machinery. A previous study in budding yeast suggests that Dpb11 controlsExpand
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Identification of the yeast MCM3-related protein as a component of xenopus DNA replication licensing factor
Replication licensing factor is thought to be involved in the strict control of the initiation of DNA replication in eukaryotes. We identified a 100 kDa protein as a candidate for the licensingExpand
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Xenopus Cdc45‐dependent loading of DNA polymerase α onto chromatin under the control of S‐phase cdk
At the onset of S phase, chromosomal replication is initiated by the loading of DNA polymerase α onto replication origins. However, the molecular mechanisms for controlling the initiation are poorlyExpand
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The phosphorylated C‐terminal domain of Xenopus Cut5 directly mediates ATR‐dependent activation of Chk1
ATR‐dependent activation of the kinase Chk1 is the initial step in signal transduction in the DNA replication checkpoint, which allows a cell to enter mitosis only after the completion of DNAExpand
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Regulation of Replication Licensing by Acetyltransferase Hbo1
ABSTRACT The initiation of DNA replication is tightly regulated in eukaryotic cells to ensure that the genome is precisely duplicated once and only once per cell cycle. This is accomplished byExpand
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Xenopus Cut5 is essential for a CDK‐dependent process in the initiation of DNA replication
Fission yeast Cut5/Rad4 and its budding yeast homolog Dpb11 are required for both DNA replication and the S‐phase checkpoint. Here, we have investigated the role of the Xenopus homolog of Cut5 in theExpand
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Central role for Cdc45 in establishing an initiation complex of DNA replication in Xenopus egg extracts
In eukaryotes, chromosomal DNA is licensed to be replicated through the sequential loading of the origin recognition complex, Cdc6 and mini‐chromosome maintenance protein complex (MCM) ontoExpand
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