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Cloning and sequence of the gene encoding a cefotaxime-hydrolyzing class A beta-lactamase isolated from Escherichia coli.
Toho-1 has shown the highest degree of similarity to K. oxytoca class A beta-lactamase, and replacement of Asn-276 by Arg with the concomitant substitution of Thr for Arg-244 is an important mutation in the extension of the substrate specificity. Expand
Zymobacter palmae gen. nov., sp. nov., a new ethanol-fermenting peritrichous bacterium isolated from palm sap
Zymobacter palmae gen. nov., sp. nov. was proposed for a new ethanol-fermenting bacterium that was isolated from palm sap in Okinawa Prefecture, Japan. The bacterium is gram-negative, facultativelyExpand
PARP-1 ensures regulation of replication fork progression by homologous recombination on damaged DNA
It is shown that PARP-1 is required for replication fork slowing on damaged DNA, and the presence of a novel checkpoint pathway in which the initiation of HR but not DNA damage delays the fork progression is supported. Expand
Molecular cloning of a putative divalent-cation transporter gene as a new genetic marker for the identification of Lactobacillus brevis strains capable of growing in beer
Northern blot analysis indicated that the hitA transcripts are expressed in cells cultivated in MRS broth supplemented withHop bitter compounds, which act as mobile-carrier ionophores, dissipating the trans-membrane pH gradient in bacteria sensitive to the hop bitter compounds by exchanging H+ for cellular divalent cations such as Mn2+. Expand
The genus Pediococcus , with notes on the genera Tetratogenococcus and Aerococcus
Pediococci are the only lactic acid bacteria that divide alternately in two perpendicular directions to form tetrads (Figure 5.1). They are invariably spherical and produce lactic acid, but no gas,Expand
Detection and identification of Brettanomyces/Dekkera sp. yeasts with a loop-mediated isothermal amplification method.
This LAMP method with primer sets for the identification of Brettanomyces/Dekkera yeasts is advantageous in terms of specificity, sensitivity and ease of operation compared with standard PCR methods. Expand
A specific oligonucleotide primer for the rapid detection of Lactobacillus lindneri by polymerase chain reaction.
Three strains, the Chinese brewery isolate DA1, the Japanese commercial beer isolate BG2, and the Japanese brewery isolate SE3, which were serologically classified as belonging to L. lindneri, were used in this study to develop a polymerase chain reaction (PCR) method for the rapid detection of the beer-spoilage heterofermentative lactic acid bacterium Lactobacillus lINDneri. Expand
Non-Denaturing Fluorescence in Situ Hybridization to Find Replication Origins in a Specific Genome Region on the DNA Fiber
Using the method designated non-denaturing FISH, this work determined the replication timing of a specific BAC clone and the standard clones, and found that at least one replication origin exists within the genomic region covered by its BACclone as an example. Expand
Identification of N-(beta-D-Glucopyranosyl)nicotinic Acid as a Major Metabolite from Niacin in Cultured Tobacco Cells
A major metabolite of niacin in an extract from cultured tobacco cells when cultured with niacin at 1 mM was analyzed by various spectroscopic and chromatographic methods, and the structure wasExpand
Isolation of human and fission yeast homologues of the budding yeast origin recognition complex subunit ORC5: human homologue (ORC5L) maps to 7q22.
This work localized the human ORC5 gene (ORC5L) to chromosome 7 using Southern and PCR analysis of DNA isolated from a panel of human/rodent somatic cell hybrids and mapped the gene locus to 7q22 using fluorescence in situ hybridization. Expand