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Transgenic banana cv. Rasthali (AAB, Silk gp) harboring Ace-AMP1 gene imparts enhanced resistance to Fusarium oxysporum f.sp. cubense race 1
TLDR
Highly regenerative embryogenic cell suspensions of cultivar Rasthali were developed using male floral meristems and significantly higher AMP protein content in transgenic lines was negatively correlated and higher root colonization was positively correlated with disease symptoms. Expand
TRANSFORMATION OF THE BANANA CULTIVAR 'NANJANGUD RASBALE' (SYN. 'RASTHALI', AAB, SILK SUBGROUP) WITH THE AMP GENE AND SCREENING FOR FUSARIUM RESISTANCE WITH A BIOASSAY
TLDR
Transgenic plants of the banana cultivar ‘Nanjangud Rasbale’ are developed by transformation with the antimicrobial peptide (AMP) gene cloned from onion seeds and with constructs developed in pCAMBIA 2301, and transformants are being further multiplied for large-scale root challenging with Foc and further selection. Expand
Development of highly regenerative embryogenic cell suspensions of 'Nanjangud Rasbale' (syn. 'Rasthali', Musa, AAB, Silk subgroup) and transformants with AMP gene
TLDR
Highly regenerative ECS cultures from this cultivar were established from floral meristems, which developed into embryos in 45 days and germinated in 60 days, and integration of the AMP gene in 10 out of 17 plants tested. Expand
Deployment of Stacked Antimicrobial Genes in Banana for Stable Tolerance Against Fusarium oxysporum f.sp. cubense Through Genetic Transformation
TLDR
The stacked lines revealed higher activity of Super Oxide Dismutase and Peroxidase compared to untransformed control which depicted higher tolerance to oxidative stress caused by Foc infection. Expand
Somatic embryogenesis and plant regeneration through cell suspension in diploid (AB) banana cultivar Elakki Bale (syn Neypoovan)
TLDR
An efficient protocol was developed using cell suspensions for somatic embryogenesis and plantlet regeneration in a most popular diploid AB banana known for its taste and keeping quality and can be used for the micropropagation and genetic manipulation. Expand
Sonication assisted Agrobacterium transformation of banana cv. Ney Poovan shoot tips with GUS reporter gene
TLDR
An efficient protocol for Agrobacterium- mediated transformation of banana cv Ney Poovan was standardized which may possibly be useful for future studies on genetic modification and development of transgenics using novel genes. Expand
RAPID AND SENSITIVE DETECTION OF XANTHOMONAS AXONOPODIS PV . PUNICAE INFECTING POMEGRANATE USING LOOP-MEDIATED ISOTHERMAL AMPLIFICATION
Bacterial blight of pomegranate caused by Xap has severely hampered pomegranate cultivation in all major pomegranategrowing states of India including Maharashtra, Karnataka, and Andhra PradeshExpand
Somatic Embryogenesis as a Tool in Genetic Transformation
TLDR
Key factors such as age of the explant, genotype, and plant growth regulators affecting the induction and regeneration of plants by embryogenic callus are discussed and various strategies to improve the transformation efficiency using somatic embryogenesis are provided. Expand
Induction of Ced9 mediated anti-apoptosis in commercial banana cultivar Rasthali for stable resistance against Fusarium wilt
TLDR
This is the first report from India on the generation of transgenic banana cultivar Rasthali expressing antiapoptotic Ced-9 gene for resistance to Fusarium wilt. Expand
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