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Scalable expansion of human pluripotent stem cells in suspension culture
- R. Zweigerdt, R. Olmer, H. Singh, A. Haverich, U. Martin
- Biology, Medicine
- Nature Protocols
- 1 April 2011
Routine commercial and clinical applications of human pluripotent stem cells (hPSCs) and their progenies will require increasing cell quantities that cannot be provided by conventional adherent… Expand
Endometrial Exosomes/Microvesicles in the Uterine Microenvironment: A New Paradigm for Embryo-Endometrial Cross Talk at Implantation
Exosomes are nanoparticles (∼100 nm diameter) released from cells, which can transfer small RNAs and mRNA via the extracellular environment to cells at distant sites. We hypothesised that exosomes or… Expand
Adhesion molecules in endometrial epithelium: tissue integrity and embryo implantation
Cell adhesion in endometrial epithelium is regulated to maintain the continuity and protectiveness of the luminal covering cell layer while permitting interstitial implantation of the embryo during a… Expand
An exploratory study of manganese exposure to welders.
Sixty welders (20 each from Plants A, B, and C) and 20 control subjects were investigated for the possible occurrence of manganese poisoning among welders exposed to the welding fumes containing this… Expand
Enzymatic cleavage of carotenoids.
Abstract 1. 1.|Carotene 15,15′-dioxygenase (EC 22.214.171.124) has been isolated from the intestine of guinea pig and rabbit and purified 38- and 30-fold, respectively, but subjecting the intestinal… Expand
Seed priming techniques in field crops-A review
Germination and seedling emergence are the critical stages in the plant life cycle. Insufficient seedling emergence and inappropriate stand establishment are the main constraints in the production of… Expand
Application of the wheat-germ cell-free translation system to produce high temperature requirement A3 (HtrA3) proteases.
Mammalian high temperature requirement A3 (HtrA3) is a serine protease of the HtrA family. It is an important factor for placental development and a tumor suppressor. The biochemical properties of… Expand
The Gaucher mouse.
Protein kinases associated with peripheral nerve myelin. 1. Phosphorylation of endogenous myelin proteins and exogenous substrates.
When highly purified myelin from rat sciatic nerve was incubated with [gamma-32P]ATP, protein components of the membrane were phosphorylated indicating the presence of both the substrate (receptor… Expand
Myelin from human peripheral nerves. Quantitative and qualitative studies in two age groups.
Myelin in femoral nerve segments obtained at autopsy was isolated quantitatively by a series of discontinuous and continuous flotation procedures. The total amount of myelin isolated from these… Expand