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Europium as a label in time-resolved immunofluorometric assays.
A nonisotopic immunoassay has been developed based on a sensitive detection of europium (III) in water solution using time-resolved fluorometry. The europium label is bound to the antibody with EDTAExpand
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New sensitive and specific assay for human immunodeficiency virus antibodies using labeled recombinant fusion protein and time-resolved fluoroimmunoassay.
A new, rapid method for the detection of human immunodeficiency virus type 1 (HIV-1) antibody by time-resolved fluoroimmunoassay (TR-FIA) was developed. In this assay format, microtitration stripsExpand
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Europium-labeled oligonucleotide hybridization probes: preparation and properties.
A chemical method for labeling of oligonucleotide probes with europium chelates is presented. A modified deoxycytidine phosphoramidite is used to introduce multiple reactive amino groups to theExpand
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Disposable roll-to-roll hot embossed electrophoresis chip for detection of antibiotic resistance gene mecA in bacteria.
We present a high-throughput roll-to-roll (R2R) manufacturing process for foil-based polymethyl methacrylate (PMMA) chips of excellent optical quality. These disposable, R2R hot embossed microfluidicExpand
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Specific Noncompetitive Immunoassay for HT-2 Mycotoxin Detection.
Here we demonstrate a novel homogeneous one-step immunoassay, utilizing a pair of recombinant antibody antigen-binding fragments (Fab), that is specific for HT-2 toxin and has a positive readout.Expand
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Time-resolved fluoroimmunoassay of human choriogonadotropin.
We describe time-resolved fluoroimmunoassay for human choriogonadotropin involving monoclonal antibodies directed against the beta- and alpha-subunits. The latter antibody was labeled with europium,Expand
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Detection of hepatitis B surface antigen using time-resolved fluoroimmunoassay
Conventional fluoroimmunoassay (FIA) methods based on various fluorescence principles have not achieved the sensitivity of radioimmunoassay (RIA) mainly because of problems of background fluorescenceExpand
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Towards automatic detection of point mutations: use of scintillating microplates in solid-phase minisequencing.
Simplification of molecular genetic techniques is one of the main features of large-scale clinical applications of mutation analysis. The solid-phase minisequencing method, which is based onExpand
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Preparation of europium-labelled DNA probes and their properties.
A chemical method for labelling DNA with a europium chelate is presented. First, primary aliphatic amino groups are introduced onto DNA in a transamination reaction. The transamination reaction isExpand
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The use of peroxidase-mediated deposition of biotin-tyramide in combination with time-resolved fluorescence imaging of europium chelate label in immunohistochemistry and in situ hybridization.
The application of europium chelates as delayed fluorescent labels in FISH and immunocytochemistry is hampered by their relatively low quantum yield. To increase the intensity of the delayedExpand
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