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Identification of endocrine-disrupting effects in aquatic vertebrates and invertebrates: report from the European IDEA project.
The findings of the present research project support the development of appropriate testing methodologies for substances with estrogenic activity, and several parameters were found to be responsive to (xeno)estrogens; however, most effects were induced only at higher, probably nonphysiological concentrations. Expand
Morphological development of the gonads in zebrafish
The sequence of gonadal alterations taking place in some of the individuals from week 5 pf onwards was interpreted to reflect the transition of protogynic ovaries into testes, which identifies zebrafish to be a juvenile hermaphrodite. Expand
Zebrafish (Danio rerio) as a model organism for investigating endocrine disruption.
  • H. Segner
  • Biology, Medicine
  • Comparative biochemistry and physiology…
  • 1 March 2009
The zebrafish has been mainly used to identify molecular targets of EDC action and to determine effect thresholds, while the potential of this model species to study immediate and delayed physiological consequences of molecular interactions has been instrumentalized only partly. Expand
Concentration- and Time-dependent Effects of the Synthetic Estrogen, 17α-ethinylestradiol, on Reproductive Capabilities of the Zebrafish, Danio rerio
The impact of estrogenic agents on zebrafish sexual development and reproductive functions as well as the reversibility of effects, varies with exposure concentration, and between partial and full life-cycle exposure. Expand
Aromatase modulation alters gonadal differentiation in developing zebrafish (Danio rerio).
Evidence is provided that exogenous compounds interfering with the P450arom system are able to disrupt, even persistently, gonadal sex differentiation of the protogynic zebrafish. Expand
An environmentally relevant concentration of estrogen induces arrest of male gonad development in zebrafish, Danio rerio.
Findings suggest that continuous estrogen exposure had arrested the developmental transition of the gonads of genetic males from the early all-ovary stage to functional testes after the exposure had ceased, however, these males apparently were able to accomplish testicular differentiation. Expand
The use of Fish Cells in Ecotoxicology: The Report and Recommendations of ECVAM Workshop 471, 2
This is the report of the forty-seventh of a series of workshops organised by the European Centre for the Validation of Alternative Methods (ECVAM), held in Angera, Italy, on 22–24 October 2001, under the co-chairmanship of Argelia Castaño and Helmut Segner. Expand
Interference of endocrine disrupting chemicals with aromatase CYP19 expression or activity, and consequences for reproduction of teleost fish.
Diverse EDCs may affect the expression and/or activity of aromatase cyp19 genes through a variety of mechanisms, many of which need further characterization in order to improve the prediction of risks posed by a contaminated environment to teleost fish population. Expand
Screening and Testing for Endocrine Disruption in Fish—Biomarkers As “Signposts,” Not “Traffic Lights,” in Risk Assessment
As research continues to develop a range of useful EDC biomarkers, environmental decision-making needs to move forward, and it is proposed that the “biomarkers as signposts” approach is a pragmatic way forward in the current risk assessment of EDCs. Expand
Isolation and primary culture of teleost hepatocytes
The review discusses procedures for isolation and primary culture of teleostean hepatocytes and the influence of different culture conditions on the physiology of the cells in vitro and suggests hepatocyte aggregate cultures are particularly promising. Expand