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Alcaligenes eutrophus CH34 is a facultative chemolithotroph with plasmid-bound resistance to heavy metals.
- M. Mergeay, D. Nies, H. Schlegel, J. Gerits, P. Charles, F. Van Gijsegem
- Biology, Medicine
- Journal of bacteriology
- 1 April 1985
Alcaligenes eutrophus strain CH34, which was isolated as a bacterium resistant to cobalt, zinc, and cadmium ions, shares with A. eutrophus strain H16 the ability to grow lithoautotrophically on… Expand
Taxonomic Study of Paracoccus denitrificans
Isolates of Paracoccus denitrificans were obtained from various habitats by enrichment in a mineral medium, using molecular hydrogen as the hydrogen donor and nitrate as the Hydrogen acceptor, and a computer analysis indicated that the strains clustered into subgroups. Expand
[A submersion method for culture of hydrogen-oxidizing bacteria: growth physiological studies].
Biology of the prokaryotes
Bacteriology paved the way to cell biology - a historical account the procaryotic cell basic prerequisites for cellular life diversity of metabolic pathways the genetics of the prokaryotes and their… Expand
Cloning of the Alcaligenes eutrophus genes for synthesis of poly-beta-hydroxybutyric acid (PHB) and synthesis of PHB in Escherichia coli.
Eight mutants of Alcaligenes eutrophus defective in the intracellular accumulation of poly-beta-hydroxybutyric acid (PHB) were isolated after transposon Tn5 mutagenesis with the suicide vector pSUP5011, and PHB-negative mutants were completely impaired in the formation of active PHB synthase, which was measured by a radiometric assay. Expand
Sulphur metabolism in Thiorhodaceae I. Quantitative measurements on growing cells ofChromatium okenii
Growth experiments and short term experiments in a stirred cuvette showed thatChromatium okenii strain Ostrau is not able to oxidize any reduced sulphur compounds except sulphide and elementary… Expand
Characterization of the inducible nickel and cobalt resistance determinant cnr from pMOL28 of Alcaligenes eutrophus CH34.
- H. Liesegang, K. Lemke, R. A. Siddiqui, H. Schlegel
- Biology, Chemistry
- Journal of bacteriology
- 1 February 1993
From pMOL28, one of the two heavy metal resistance plasmids of Alcaligenes eutrophus strain CH34, an EcoRI-PstI fragment was cloned into plasmid pVDZ'2 and mutations located upstream of cnrC resulted in various phenotypic changes, which resulted in two- to fivefold-increased nickel resistance in both hosts. Expand
Combined nickel-cobalt-cadmium resistance encoded by the ncc locus of Alcaligenes xylosoxidans 31A.
The nickel-cobalt-cadmium resistance genes carried by plasmid pTOM9 of Alcaligenes xylosoxidans 31A are located on a 14.5-kb BamHI fragment and the nucleotide sequence of the ncc locus revealed seven open reading frames designated nccYXHCBAN. Expand
Purification and properties of soluble hydrogenase from Alcaligenes eutrophus H 16.
The soluble hydrogenase from Alcaligenes eutrophus H 16 was purified 68-fold with a yield of 20% and a final specific activity (NAD reduction) of about 54 mumol H2 oxidized/min per mg protein, and was shown to be homogenous by polyacrylamide gel electrophoresis. Expand
The isolation of mutants not accumulating poly-β-hydroxybutyric acid
SummaryFive mutant strains of Hydrogenomonas H 16 which synthesize poly-β-hydroxybutyric acid either slowly or not at all have been isolated following nitrite and NMG treatment of wild type cells.… Expand