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Alternatives to the use of fetal bovine serum: human platelet lysates as a serum substitute in cell culture media.
TLDR
Growth experiments, viability assays, and platelet lysate-induced activation of ERK1/2 mitogen-activated protein kinase revealed platelets lysates as a valuable alternative to FBS in cell culture media. Expand
Cross-Linking of CD32 Induces Maturation of Human Monocyte-Derived Dendritic Cells Via NF-κB Signaling Pathway1
TLDR
It is shown that cross-linking of FcγR-type II (CD32) with immobilized IgG (imIgG) can induce maturation of human monocyte-derived DC via the NF-κB signaling pathway and that DC, which matured in the presence of immune complexes, may contribute to prevention of pathological immune responses. Expand
Simultaneous measurement of serum tryptophan and kynurenine by HPLC.
TLDR
A new HPLC method is described to determine the amounts of kynurenine and tryptophan in serum simultaneously with use of an external albumin-based calibrator and an internal calibrator, enabling indirect examination of endogenous interferon-γ formation. Expand
Atorvastatin suppresses interferon‐γ‐induced neopterin formation and tryptophan degradation in human peripheral blood mononuclear cells and in monocytic cell lines
TLDR
It is demonstrated that atorvastatin directly inhibits IFN‐γ‐mediated pathways in monocytic cells, suggesting that both immunoreactivity of T cells and of monocyte‐derived macrophages are down‐regulated by this statin. Expand
Regulatory interactions between iron and nitric oxide metabolism for immune defense against Plasmodium falciparum infection.
TLDR
The effect of DFO on the clearance of plasmodia appears to be due to enhanced generation of NO, rather than to limitation of iron availability to the parasite. Expand
Longitudinal study of tryptophan degradation during and after pregnancy.
TLDR
It is concluded that IDO is activated in pregnancy and that the decrease of tryptophan might be related to immune activation phenomena, and sustained increase of kynurenine postpartum seems independent from immune activation process. Expand
Monocyte‐derived dendritic cells release neopterin
TLDR
The results showed that IFN‐γ as well as LPS are inducers of IDO in DC, and neopterin production in DC was highly sensitive toIFN‐α and IFn‐β. Expand
Aspirin down‐regulates tryptophan degradation in stimulated human peripheral blood mononuclear cells in vitro
TLDR
The parallel influence of aspirin on both biochemical pathways implies that there was no direct inhibitory effect of aspirinon IDO; rather, it inhibits production of IFN‐γ in mitogen‐treated PBMC. Expand
Moderate hyperhomocysteinemia and immune activation.
TLDR
Moderate hyperhomocysteinemia is associated with an increased risk of atherosclerosis, thrombosis and neurodegenerative diseases, and the question is addressed, whether effects attributed to homocysteine, are not rather the consequence of folate and vitamin B12-deficiency. Expand
In vitro testing for anti-inflammatory properties of compounds employing peripheral blood mononuclear cells freshly isolated from healthy donors
TLDR
The determination of tryptophan degradation and neopterin production in PBMC reflects various pro- and anti-inflammatory cascades that are of relevance also in patients and may improve throughput, speed and cost-effectiveness in drug discovery. Expand
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