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Induction of pluripotent stem cells from mouse embryonic fibroblasts by Oct4 and Klf4 with small-molecule compounds.
A small-molecule combination, BIX-01294 and BayK8644, is identified that enables reprogramming of Oct4/Klf4-transduced mouse embryonic fibroblasts, which do not endogenously express the factors essential for reprograming. Expand
A combined chemical and genetic approach for the generation of induced pluripotent stem cells.
Two approaches toward identifying conditions that can replace viral transduction of oncogenic transcription factors (TFs) and enhance reprogramming efficiency are explored, with one finding that neural progenitor cells can be reprogrammed with fewer genetic manipulations than previously reported somatic cells. Expand
A chemical platform for improved induction of human iPSCs
A chemical approach is described that dramatically improves the efficiency of iPSC generation from human fibroblasts, within seven days of treatment, which will provide a basis for developing safer, more efficient, nonviral methods for reprogramming human somatic cells. Expand
Revealing a core signaling regulatory mechanism for pluripotent stem cell survival and self-renewal by small molecules
It is shown that the primary cause of hESC death following enzymatic dissociation comes from an irreparable disruption of E-cadherin signaling, which then leads to a fatal perturbation of integrin signaling, and the resulting survival of ESCs were controlled by specific growth factor signaling. Expand
Identification of carbohydrate anomers using ion mobility–mass spectrometry
It is demonstrated that ion mobility–mass spectrometry—a method that separates molecules according to their mass, charge, size, and shape—can unambiguously identify carbohydrate linkage-isomers and stereoisomers, and could have an impact on the field of carbohydrate synthesis similar to that of the advent of high-performance liquid chromatography on the fields of peptide assembly in the late 1970s. Expand
Automated solid-phase synthesis of chondroitin sulfate glycosaminoglycans.
A novel approach for automated solid-phase synthesis of GAG oligosaccharides that is based in part on established methods for generating the glycan portion of glycoproteins and glycolipids is described. Expand
A semisynthetic Streptococcus pneumoniae serotype 8 glycoconjugate vaccine
A medicinal chemistry strategy based on a combination of automated glycan assembly, glycan microarray–based monoclonal antibody (mAb) reverse engineering, and immunological evaluation in vivo to uncover a protective glycan epitope (glycotope) for S. pneumoniae serotype 8 (ST8). Expand
Pushing the limits of automated glycan assembly: synthesis of a 50mer polymannoside.
A suitable mannose building block is identified and a capping step is applied in the final five AGA cycles to minimize (n - 1) deletion sequences that are otherwise difficult to remove by HPLC. Expand
Microbe-focused glycan array screening platform
This work presents an extensive synthetic glycan collection focused on microbial glycans as a tool to investigate microbial glycobiology and presents a microbe-focused glycan microarray platform based on oligosaccharides obtained by chemical synthesis. Expand
Automated Glycan Assembly of Oligo-N-Acetyllactosamine and Keratan Sulfate Probes to Study Virus-Glycan Interactions
Summary Oligo- N -acetyllactosamine (LacNAc) and keratan sulfate (KS) glycans exert crucial functions in disease-relevant processes, including cancer formation, inflammation, and viral infection. ToExpand