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The measurement of membrane potential and deltapH in cells, organelles, and vesicles.
The chapter describes methods for determining the membrane potential in suspensions of cells, organelles, or vesicles, and the value of the proton-motive force is important for an experimental evaluation of the chemiosmotic hypothesis. Expand
Atovaquone, a Broad Spectrum Antiparasitic Drug, Collapses Mitochondrial Membrane Potential in a Malarial Parasite*
A flow cytometric assay is developed as an alternate means to study mitochondrial functions in intact erythrocytes infected with Plasmodium yoelii, a rodent malaria parasite that suggests that atovaquone, shown to inhibit mitochondrial electron transport, also depolarizes malarial mitochondria with consequent cellular damage and death. Expand
Quantitative assay by flow cytometry of the mitochondrial membrane potential in intact cells.
A new protocol is developed in which cells are equilibrated with very low dye concentrations (<1 nM), and under these condition, the cell fluorescence appears to be correlated with the magnitude of DeltaPsim, as evident from the sensitivity of the fluorescence to low concentrations of uncouplers, ionophores and inhibitors of the mitochondrial proton pumps. Expand
Membrane potential and surface potential in mitochondria: Uptake and binding of lipophilic cations
  • H. Rottenberg
  • Chemistry, Medicine
  • The Journal of Membrane Biology
  • 2005
The values of Δψ estimated from this equation are in remarkably good agreement with those estimated from the distribution of86Rb in the presence of valinomycin. Expand
The proton electrochemical gradient in Escherichia coli cells.
A similar pattern of dependence of delta pH on extracellular pH was observed in EDTA/valinomycin-treated cells but the delta psi was almost constant over the whole range of extrace cellular pH values (6-8) implying electroneutral proton movement. Expand
Adenine nucleotides regulate Ca2+ transport in brain mitochondria
Analysis of the effects of various inhibitors on Ca2+ uptake and efflux suggest that locking the ADP/ATP translocator in its M‐state stimulates electrogenic Ca2- uptake and, to a lesser extent, inhibits Ca2+, and it is suggested that this effect is due to a modulation of the surface charge on the M‐side which enhances Ca2+. Expand
Determination of ΔpH in Chloroplasts
In sub-chloroplast particles the light-induced Δ pH determined by this method was only somewhat lower than in chloroplasts, and inhibition of phosphorylation by methylamine in these particles was parallel to that of the ΔpH. Expand
Relation between the gradient of the ATP/ADP ratio and the membrane potential across the mitochondrial membrane.
It is concluded that under these conditions the ATP-ADP exchange is largely electrical. Expand
31P nuclear magnetic resonance studies of bioenergetics and glycolysis in anaerobic Escherichia coli cells.
It is concluded that ATP produced by glycolysis is hydrolyzed by the membrane ATPase to generate a delta pH and that FCCP stimulates ATP hydrolysis by ATPase and collapses the proton gradient. Expand