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Proton magnetic resonance ttudies of the interactions of histones F1 and F2B with DNA.
The characterization of two different regions led to the suggestion that the basic regions of the polypeptide chains are the primary sites of interaction with DNA, while the non-basic regions have the potential for the formation of definite conformations and might be involved in specific interactions other than with DNA. Expand
The formation of dihydrodiols by the chemical or enzymic oxidation of benz[a] anthracene and 7,12-dimethylbenz[a] anthracene.
The structures and sterochemistry of the dihydrodiols were established by comparisons of their UV spectra and chromatographic characteristics using HPLC with those of authentic compounds or, when no authentic compounds were available, by UV, NMR and mass spectral analysis. Expand
Magnetic Resonance Studies of Deoxyribonucleoprotein
Of the five major types of histone fraction normally associated with mammalian genetic material, the lysine-rich histone F1 (or histone I) has some properties which set it apart from the others, and a proposed scheme for this interaction, involving an antiparallel alignment of chain segments has been presented. Expand
Spectroscopic studies of the conformations of histones and protamine
Abstract Earlier optical rotatory dispersion studies on calf thymus histone fractions showed that increasing the salt molarity of aqueous solution caused an increase in the optical rotatoryExpand
Studies on the role and mode of operation of the very-lysine-rich histone H1 (F1) in eukaryote chromatin. The conformation of histone H1.
Ionic, hydrogen-bond and hydrophobic interactions all appear to contribute to the stability of the structure, which is predicted to contain alpha-helices in regions 42--55 and 58--75 and no evidence was found for beta-structures, either inter or intramolecular, or for any structure formation outside the region 40--115. Expand
A conformational study of glucagon.
It appears that glucagon possesses no difined tertiary structure in solution, but may be regarded as being in a state of α-helix ⇌ random coik equilibrium, near the high-temperature end of the transition range. Expand
Simple computer-aided approach for the analyses of the nuclear-magnetic-resonance spectra of histones. Fractions F1, Fsa1, F2B, cleaved halves of F2B and F2B-DNA.
A simple method for the computer simulation of high-resolution nuclear magnetic resonance spectra is described and applied to the analyses of salt-induced changes in the spectra of histones, which allows the information contained in the spectral envelope to be used and has led to some modifications of the earlier proposals of the segments of histone involved in salt- induced conformational changes, interhistone interactions and interactions with DNA. Expand
Conformations and interactions of histone H2A (F2A2, ALK).
Comparison of observed with simulated NMR spectra has led to the proposal that an ionic strength dependent equilibrium exists between largely unstructured coil molecules and fully structured and aggregated molecules. Expand