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Global trends of whole-genome duplications revealed by the ciliate Paramecium tetraurelia
TLDR
It is shown that in the unicellular eukaryote Paramecium tetraurelia, a ciliate, most of the nearly 40,000 genes arose through at least three successive whole-genome duplications.
Ultrastructural Organization of Bovine Chromaffin Cell Cortex—Analysis by Cryofixation and Morphometry of Aspects Pertinent to Exocytosis
TLDR
Analysis of ultrathin sections from isolated bovine chromaffin cells grown on plastic support, after fast freezing, by quantitative electron microscopy determined the size and intracellular distribution of dense core vesicles (DVs or Chromaffin granules) and of clear vesicle (CVs) and found that DVs appear randomly packed inside cells.
Glycosylphosphatidyl inositol-anchored proteins and fyn kinase assemble in noncaveolar plasma membrane microdomains defined by reggie-1 and -2.
TLDR
Reggie-1 and -2 define raft-related microdomain signaling centers in neurons and T cells, and the protein complex involved in signaling becomes subject to degradation.
Identification of reggie-1 and reggie-2 as plasmamembrane-associated proteins which cocluster with activated GPI-anchored cell adhesion molecules in non-caveolar micropatches in neurons.
Neurons are believed to possess plasmalemmal microdomains and proteins analogous to the caveolae and caveolin of nonneuronal cells. Caveolae are plasmalemmal invaginations where activated
An Ins(1,4,5)P3 receptor in Paramecium is associated with the osmoregulatory system
TLDR
It is proposed here that Ins(1,4,5)P3 receptors serve a new function, i.e. a latent, graded reflux of Ca2+ to fine-tune [Ca2+] homeostasis.
PrPc capping in T cells promotes its association with the lipid raft proteins reggie‐1 and reggie‐2 and leads to signal transduction
TLDR
PrPc association with reggie rafts triggers distinct transmembrane signal transduction events in T cells that promote the focal concentration of PrPc itself by guiding activated PrPC into preformed reggie caps and then to the recruitment of important interacting signaling molecules.
Quenched flow analysis of exocytosis in Paramecium cells: time course, changes in membrane structure, and calcium requirements revealed after rapid mixing and rapid freezing of intact cells
TLDR
It is concluded that no Ca++ influx is necessary for induction of membrane fusion, and a quenched flow device for rapid mixing and rapid freezing of cells without impairment is developed.
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