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Activation of follicle development: the primordial follicle.
  • H. Picton
  • Biology, Medicine
  • Theriogenology
  • 1 April 2001
The development of cell culture systems that use primordial follicles as a source of oocytes for in vitro growth and maturation will enable us to maximize the potential of high genetic merit females and to shorten generation intervals. Expand
The molecular basis of oocyte growth and development
Throughout oocyte development follicle cell support is fundamental to provide the germ line cell with nutrients and growth regulators to ensure progression through the protracted growth phase, and the oocyte actively promotes growth and differentiation of the follicular cells. Expand
A European perspective on testicular tissue cryopreservation for fertility preservation in prepubertal and adolescent boys.
An algorithm for the cryopreservation of sperm and testicular tissue is proposed for prepubertal boys and adolescent males at high risk of fertility loss and after consideration of the relevant ethical and legal challenges. Expand
Expression of mRNAs for DNA methyltransferases and methyl‐CpG‐binding proteins in the human female germ line, preimplantation embryos, and embryonic stem cells
Monitoring of the expression of the mRNAs of DNA methyltransferases, methyl‐CpG‐binding domain proteins, and CpG binding protein in a developmental series of amplified cDNA samples derived from staged human ovarian follicles, oocytes, preimplantation embryos, human embryonic stem (hES) cells and in similar murine c DNA samples demonstrated an association of the DNMT3b1 transcript variant with totipotent and pluripotent human cells. Expand
Current achievements and future research directions in ovarian tissue culture, in vitro follicle development and transplantation: implications for fertility preservation.
Experiments on ovarian tissue from non-human primate models and from consenting fertile and infertile patients benefit from a multidisciplinary approach. Expand
The in vitro growth and maturation of follicles.
In large animals and humans, the complete in vitro growth and maturation of oocytes is only likely to be achieved following the development of a multistage strategy that closely mimics the ovary in vivo, and this approach will be initiated in situ by the culture of ovarian cortex. Expand
Nutrient concentrations in murine follicular fluid and the female reproductive tract.
The concentrations of glucose, pyruvate, lactate and 19 amino acids in murine preovulatory follicles and oestrous oviductal and uterine fluids determined extend the understanding of cellular metabolism and of nutritional environments of the oocyte and early embryo as they progress along the reproductive tract in vivo. Expand
Mutations causing familial biparental hydatidiform mole implicate c6orf221 as a possible regulator of genomic imprinting in the human oocyte.
The previously described biological properties of their respective gene families suggest that NLRP7 and C6orf221 may interact as components of an oocyte complex that is directly or indirectly required for determination of epigenetic status on the oocyte genome. Expand
Isolation, characterization and expression of the human Factor In the Germline alpha (FIGLA) gene in ovarian follicles and oocytes.
The identification of the human homologue for this gene which maps to chromosome 2p12 is confirmed and is required to understand its role in normal human oocyte development and the potential involvement in human infertility. Expand
Expression of 11 members of the BCL‐2 family of apoptosis regulatory molecules during human preimplantation embryo development and fragmentation
This study addresses the importance of examining single human embryos representative of the viable population for a large number of genes, in order to establish meaningful expression profiles and provide information on overlapping function in a large gene family. Expand