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Polyethylenimine-graft-poly(ethylene glycol) copolymers: influence of copolymer block structure on DNA complexation and biological activities as gene delivery system.
The degree of PEGylation and the MW of P EG were found to strongly influence DNA condensation of PEI and therefore also affect the biological activity of the PEI-g-PEG/DNA complexes, providing a basis for the rational design of block copolymer gene delivery systems.
PEGylation of poly(ethylene imine) affects stability of complexes with plasmid DNA under in vivo conditions in a dose-dependent manner after intravenous injection into mice.
The data demonstrate that PEG-PEI used in this study is not suitable for low dose gene delivery, however, polyplex stability is similar to PEI/pDNA combined with a more favorable organ deposition and significantly lower acute in vivo toxicity.
Synthesis, Characterization, and Biocompatibility of Polyethylenimine-graft-poly(ethylene glycol) Block Copolymers
Two series of block copolymers were prepared by grafting linear poly(ethylene glycol) (PEG) onto branched polyethylenimine (PEI). In the first series, the PEI (25 000) was grafted with varied numbers
The Structure of PEG-Modified Poly(Ethylene Imines) Influences Biodistribution and Pharmacokinetics of Their Complexes with NF-κB Decoy in Mice
A sufficiently high density of PEG molecules is necessary to effectively prevent opsonization and thereby rapid clearance from blood stream.
Pegylated polyethylenimine-Fab' antibody fragment conjugates for targeted gene delivery to human ovarian carcinoma cells.
The advantage of the PEG-PEI-Fab' conjugated to the antigen binding fragment (Fab') of the OV-TL16 antibody is its specificity, which was demonstrated by competition experiments with free Fab' in the cell culture media during transfection experiments and by using OA3-negative cells.
Star-shaped poly(ethylene glycol)-block-polyethylenimine copolymers enhance DNA condensation of low molecular weight polyethylenimines.
Star-shaped poly(ethylene glycol)-block-polyethylenimine [star-(PEG-b-PEI] significantly enhance plasmid DNA condensation of low molecular weight (MW) PEIs and exhibited aDNA condensation potential as high as high MW PEI.
Physicochemical and biological characterization of polyethylenimine-graft-poly(ethylene glycol) block copolymers as a delivery system for oligonucleotides and ribozymes.
PEGylated PEI block copolymers represent a promising new class of drug delivery systems for ODN and ribozymes with increased biocompatibility and physical stability.
Effect of poly(ethylene imine) molecular weight and pegylation on organ distribution and pharmacokinetics of polyplexes with oligodeoxynucleotides in mice.
Although PEGylated PEI demonstrated a slower (125)I-uptake into the RES organs compared with 25-kDa PEI due to the shielding effect of PEG [poly(ethylene glycol), it was not able to better stabilize the complexes in the circulation or protect DNA from degradation.