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Analysis of the transfer region of the Streptomyces plasmid SCP2*
The deduced sequences of the putative TraA and TraB proteins showed no overall similarity to known protein sequences, but the phenotype of traA mutant plasmids and sequence motifs in the putatives TraA protein suggested that it might be a DNA helicase. Expand
Identification, Cloning, Expression, and Characterization of the Extracellular Acarbose-Modifying Glycosyltransferase, AcbD, from Actinoplanes sp. Strain SE50
The function of the enzyme in the extracellular formation of the series of acarbose-homologous compounds produced by Actinoplanes sp. Expand
Isolation of mak1 from Actinoplanes missouriensis and evidence that Pep2 from Streptomyces coelicolor is a maltokinase
Sequence comparisons demonstrate that mak1‐related genes coding for homologous proteins are present in many bacterial genomes including taxonomic distantly related groups such as Rhodospirillales or green sulfur bacteria. Expand
Isolation and characterization of maltokinase (ATP:maltose 1-phosphotransferase) from Actinoplanes missouriensis
Tryptic peptides of the enzyme were sequenced, and the sequences of these peptides will allow construction of degenerate primers to identify the gene coding for this unique kinase. Expand
Three trehalose synthetic pathways in the acarbose-producing Actinoplanes sp. SN223/29 and evidence for the TreY role in biosynthesis of component C
It is suggested that the mutation of treY can improve acarbose production by repressing component C production by repelling isomerase activity in Actinoplanes sp. Expand
Studies on the biosynthesis of the alpha-glucosidase inhibitor acarbose: valienamine, a m-C7N unit not derived from the shikimate pathway.
Feeding experiments with Actinoplanes sp. SN223/29 showed that 3-amino-5-hydroxy-[7-13C]benzoic acid is not incorporated into acarbose (I). The valienamine moiety of I is thus not derived in the sameExpand
Maltokinase (ATP: maltose 1‐phosphotransferase) from Actinoplanes sp.: demonstration of enzyme activity and characterization of the reaction product
Cell free extract of the acarbose producer Actinoplanes sp. catalyzes ATP‐dependent phosphorylation of maltose. This was shown by two different assays. The product was purified and its structureExpand
Acarbose 7-phosphotransferase from Actinoplanes sp.: purification, properties, and possible physiological function.
A phosphotransferase which modifies the alpha-glucosidase inhibitor acarbose by phosphorylation at its 7-position was isolated from the acarbose producer Actinoplanes sp. and purified to homogeneity.Expand
The Genome of φAsp2, an Actinoplanes Infecting Phage
The first genome of a virus infecting a representative of the eubacterial genus Actinoplanes is presented and the unique overall structure of the φAsp2-genome does not bear resemblance to any other known viral genome. Expand
Production of tylosin and nikkomycin by immobilized Streptomyces cells
  • M. Veelken, H. Pape
  • Chemistry
  • European journal of applied microbiology and…
  • 1 December 1982
SummaryMycelia of Streptomyces sp. T 59-235 and Streptomyces tendae Tü 901 (producing the antibiotics tylosin and nikkomycin, resp.) were immobilized in different carriers. With both organisms bestExpand