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Accumulation of mercury in transgenic tobacco expressing bacterial polyphosphate.
TLDR
The results suggest that the integrated ppk gene, encoding polyphosphate kinase (PPK), a key enzyme for polyP biosynthesis, is stably conserved in tobacco genome, and translated to active PPK which catalyzed biosynthesis of polyP, and suggest that polyP is capable of reducing the cytotoxicity of Hg2+, probably via chelation formation with polyP. Expand
Nucleotide sequence and expression of the organomercurial-resistance determinants from a Pseudomonas K-62 plasmid pMR26.
TLDR
Induction experiments and maxicell analyses of the mer-polypeptides revealed that pMRA17 mer operon expressed mercurial-inducible phenotype and the merB and merE as well as the merA were under the control of MerR which could activate not only by mercuric ion but also by organomercurials. Expand
The merG gene product is involved in phenylmercury resistance in Pseudomonas strain K-62.
TLDR
The results demonstrate that the merG gene is involved in phenylmercury resistance, presumably by reducing in-cell permeability to phenyl MerG, and suggest that the mature MerG protein may be located in the periplasm. Expand
Genetic Engineering of Bacteria for Environmental Remediation of Mercury
TLDR
The mercury accumulation system was designed so that overexpressed polyphosphate would serve as a mercury accumulator; the mercury transport system would make the bacterial cell specifically accumulate mercury; and the intracellular accumulation process would allow the bioaccumulation system to be less sensitive to ambient conditions. Expand
Hepatic changes in adenine nucleotide levels and adenosine 3'-monophosphate forming enzyme in streptozotocin-induced diabetic mice.
TLDR
The results obtained suggested that change in the mitochondrial 3'-AMP forming enzyme activity might reflect the pathophysiological change of mitochondrial function with the development of diabetes. Expand
Engineering expression of bacterial polyphosphate kinase in tobacco for mercury remediation
TLDR
The expression of ppk gene in transgenic tobacco plants might provide a means for phytoremediation of mercury pollution, and the results suggest that ppk-specified polyphosphate has abilities to reduce mercury toxicity, probably via chelation mechanism, and also to accumulate mercury in the transgenic Tobacco. Expand
Polyphosphate produced in recombinant Escherichia coli confers mercury resistance.
TLDR
Results suggest that the synthesis of polyphosphate as well as the expression of the mer genes is mercury-inducible and regulated by merR, and indicate that poly PHosphate is capable of reducing the cytotoxicity of the transported Hg2+ probably via chelation between polyph phosphate and Hg 2+. Expand
The MerE protein encoded by transposon Tn21 is a broad mercury transporter in Escherichia coli
TLDR
It is suggested for the first time that the merE gene is a broad mercury transporter mediating the transport of both CH3Hg(I) and Hg(II) across the bacterial membrane. Expand
A spider toxin (JSTX) inhibits l-glutamate uptake by rat brain synaptosomes
TLDR
It is clear that JSTX has the ability to inhibit not only L-glutamate binding to its synaptic membrane receptors but also L- glutamate uptake by synaptosomes, and that polyamines linked to 2,4-DHPA-ASN in the molecule of spider toxins may participate in the inhibition of L- GLUTamate uptake. Expand
Role of MerC, MerE, MerF, MerT, and/or MerP in resistance to mercurials and the transport of mercurials in Escherichia coli.
TLDR
It is demonstrated for the first time that in addition to MerE and MerT, MerF and MerC are broad-spectrum mercury transporters that mediate both Hg(II) and phenylmercury transport into cells. Expand
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