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Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection
TLDR
These mutants—the ‘Keio collection’—provide a new resource not only for systematic analyses of unknown gene functions and gene regulatory networks but also for genome‐wide testing of mutational effects in a common strain background, E. coli K‐12 BW25113.
Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research.
TLDR
A complete set of cloned individual genes encoding Histidine-tagged proteins with or without GFP fused for functional genomic analysis of Escherichia coli K-12 strain should provide unique resources for systematic functional genomic approaches.
Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110
TLDR
Direct DNA sequencing of PCR products for the 251 regions with short indel and base disparities revealed that only eight sites are true differences, and errors in the original MG1655 sequence were mostly within portions sequenced with out‐dated technology based on radioactive chemistry.
Two‐component system VraSR positively modulates the regulation of cell‐wall biosynthesis pathway in Staphylococcus aureus
TLDR
The observation strongly indicates that VraSR constitutes a positive regulator of cell‐wall peptidoglycan synthesis, and that is deeply involved in the expression of β‐lactam and glycopeptide resistance in S. aureus.
Transcriptome analysis of all two‐component regulatory system mutants of Escherichia coli K‐12
TLDR
Results support the view that a network of functional interactions, such as cross‐regulation, exists between different two‐component systems of Escherichia coli.
Escherichia coli K-12: a cooperatively developed annotation snapshot—2005
TLDR
A snapshot analysis based on the most recent genome sequences of two E.coli K-12 strains allows comparison of their genotypes and mutant status of alleles.
Genome Structure of the Legume, Lotus japonicus
TLDR
Structural features of the L. japonicus genome are reported, providing the first opportunity to look into the complex and unique genetic system of legumes.
Regulation of the heat-shock response in bacteria.
TLDR
A clue to further understanding of early regulatory events came from recent analysis of translational induction and subsequent shut-off of sigma 32 synthesis, which indicates that a distinct segment of s Sigma 32 polypeptide further downstream is involved in the DnaK/DnaJ-mediated Shut-off and destabilization ofSigma 32 that may be mutually interconnected.
Multiple High-Throughput Analyses Monitor the Response of E. coli to Perturbations
TLDR
E. coli seems to use complementary strategies that result in a metabolic network robust against perturbations, and actively regulated enzyme levels to maintain a stable metabolic state in response to changes in growth rate.
Genome-Wide Analyses Revealing a Signaling Network of the RcsC-YojN-RcsB Phosphorelay System in Escherichia coli
TLDR
Evidence is obtained which supports the view that there is a signaling network that connects the Rcs system with the PhoQ/P system, which coordinately regulates extracellular polysaccharide synthesis in response to the external concentrations of divalent cations.
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