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Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection
We have systematically made a set of precisely defined, single‐gene deletions of all nonessential genes in Escherichia coli K‐12. Open‐reading frame coding regions were replaced with a kanamycinExpand
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Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research.
  • M. Kitagawa, T. Ara, +4 authors H. Mori
  • Biology, Medicine
  • DNA research : an international journal for rapid…
  • 9 January 2006
Based on the genomic sequence data of Escherichia coli K-12 strain, we have constructed a complete set of cloned individual genes encoding Histidine-tagged proteins with or without GFP fused forExpand
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Two‐component system VraSR positively modulates the regulation of cell‐wall biosynthesis pathway in Staphylococcus aureus
DNA microarray covering the whole genome of Staphylococcus aureus strain N315 was prepared to investigate transcription profiles. The microarray analyses revealed that vancomycin inducesExpand
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Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110
With the goal of solving the whole‐cell problem with Escherichia coli K‐12 as a model cell, highly accurate genomes were determined for two closely related K‐12 strains, MG1655 and W3110. CompletionExpand
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Transcriptome analysis of all two‐component regulatory system mutants of Escherichia coli K‐12
We have systematically examined the mRNA profiles of 36 two‐component deletion mutants, which include all two‐component regulatory systems of Escherichia coli, under a single growth condition. DNAExpand
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Escherichia coli K-12: a cooperatively developed annotation snapshot—2005
The goal of this group project has been to coordinate and bring up-to-date information on all genes of Escherichia coli K-12. Annotation of the genome of an organism entails identification of genes,Expand
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Genome Structure of the Legume, Lotus japonicus
The legume Lotus japonicus has been widely used as a model system to investigate the genetic background of legume-specific phenomena such as symbiotic nitrogen fixation. Here, we report structuralExpand
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Multiple High-Throughput Analyses Monitor the Response of E. coli to Perturbations
Analysis of cellular components at multiple levels of biological information can provide valuable functional insights. We performed multiple high-throughput measurements to study the response ofExpand
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Large-scale identification of protein-protein interaction of Escherichia coli K-12.
Protein-protein interactions play key roles in protein function and the structural organization of a cell. A thorough description of these interactions should facilitate elucidation of cellularExpand
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Genome-wide analyses revealing a signaling network of the RcsC-YojN-RcsB phosphorelay system in Escherichia coli.
In Escherichia coli, capsular colanic acid polysaccharide synthesis is regulated through the multistep RcsC-->YojN-->RcsB phosphorelay. By monitoring a hallmarked cps::lacZ reporter gene, we firstExpand
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