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High‐yield secretion of recombinant gelatins by Pichia pastoris
TLDR
Recombinant non‐hydroxylated gelatins based on mouse type I and rat type III collagen sequences were secreted from the methylotrophic yeast Pichia pastoris, using the Saccharomyces cerevisiae α‐mating factor prepro signal to represent the highest level of heterologous protein secretion reported to date for P. pastoris.
Cloning, expression and characterisation of two tyrosinase cDNAs from Agaricus bisporus
TLDR
Alignment and phylogenetic analysis of 35 tyrosinase and polyphenol oxidase sequences of animal, plant, fungal, and bacterial origin indicated conserved copper-binding domains, and stronger conservation within genera than between them.
Particle bombardment and the genetic enhancement of crops: myths and realities
TLDR
In this review, recent data highlighting the positive impact of particle bombardment on the genetic transformation of plants is discussed, focusing on the fate of exogenous DNA, its organization and its expression in the plant cell.
Alternative sources of natural rubber
TLDR
A complementary approach to guayule with yeast species is set up, which may more quickly show the applicability and relevance of genes selected and the risk of crop failure, diminishing acreage, and other disadvantages outlined below.
Transgene Organisation in Potato After Particle Bombardment-mediated (co-)Transformation Using Plasmids and Gene Cassettes
TLDR
Protocols for efficient co-transformation of potato internodes with genes contained in separate plasmids or gene cassettes using particle bombardment were established, and plants containing the gusA reporter-gene and the nptII selectable-marker have been characterised with respect to the molecular organisation of the donor DNAs.
Bio-refinery as the bio-inspired process to bulk chemicals.
This paper describes several examples of knowledge-intensive technologies for the production of chemicals from biomass, which take advantage of the biomass structure in a more efficient way than the
A system of categorizing enzyme–cell wall associations in Agaricus bisporus, using operational criteria
TLDR
It is thought that the categorization presented could be of general applicability in fungi as well as in higher plants to specify enzyme–wall associations in a straightforward, comparable manner, thus avoiding some of the ambiguous terms prevailing in the literature, such as "weakly", "strongly" or "tightly" wall bound.
The Crystallographic Structure of the Mannitol 2-Dehydrogenase NADP+ Binary Complex from Agaricus bisporus *
TLDR
The crystal structure of MtDH was solved at 1.5-Å resolution and refined to an R value of 19.3%.
Utilisation of saccharides in extruded domestic organic waste by Clostridium acetobutylicum ATCC 824 for production of acetone, butanol and ethanol
TLDR
The results indicate that growth and ABE production on DOW is mainly supported by soluble saccharides in the medium.
Cloning and Characterization of NADP-Mannitol Dehydrogenase cDNA from the Button Mushroom, Agaricus bisporus, and Its Expression in Response to NaCl Stress
TLDR
Phylogenetic analysis based on amino acid sequences from mannitol(-1-phosphate) dehydrogenases indicated a close relationship between the substrate specificity of the enzymes and phylogenetic differentiation.
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