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ECA, the enterobacterial common antigen.
Enterobacterial common antigen (ECA) is a family-specific surface antigen shared by all members of the Enterobacteriaceae and is restricted to this family. It is found in freshly isolated wild-typeExpand
Brucella abortus 16S rRNA and lipid A reveal a phylogenetic relationship with members of the alpha-2 subdivision of the class Proteobacteria
The chemical analysis of the lipid A fraction revealed that Brucella species contain both glucosamine and diaminoglucose, thus suggesting the presence of a so-called mixed lipid A type, and the serological analysis with polyclonal and monoclonal antibodies is in agreement with the existence of mixed lipid B type in B. abortus. Expand
Bacterial lipopolysaccharides
Lipopolysaccharides a re t h e 0-antigens and the endotoxins of Gramnegative bacteria. They a r e localized in the outer membrane of t h e bacterial ce l l wall and play an important role in t h eExpand
Occurrence of lipid A variants with 27-hydroxyoctacosanoic acid in lipopolysaccharides from members of the family Rhizobiaceae
The distribution of 27-hydroxyoctacosanoic acid as well as analysis of lipid A backbone sugars revealed the taxonomic relatedness of various strains of the Rhizobiaceae. Expand
Different sugar compositions of lipopolysaccharides isolated from phase I and pure phase II cells of Coxiella burnetii
The compositions of lipopolysaccharides I and II, isolated from Coxiella burnetii phase I and pure phase II cells, respectively, showed drastic and clear-cut differences, and the similarity of the phase variation in C. burningetii to the well-known smooth-to-rough mutation found in Enterobacteriaceae is discussed. Expand
Isolation and chemical characterization of the enterobacterial common antigen.
The isolation of the soluble form of the Enterobacteriaceae common antigen from Salmonella montevideo was achieved by a combination of the phenol/water method and the Phenol/chloroform/petroleum ether extraction procedure and the presence of additional still unknown lipid components which might account for the missing 30% and for the low solubility of the isolated antigen in water is discussed. Expand
Biosynthesis of enterobacterial common antigen in Escherichia coli. Biochemical characterization of Tn10 insertion mutants defective in enterobacterial common antigen synthesis.
Biochemical characterization and re-examination of a mutant possessing the rff-726 lesion revealed that it was defective in the synthesis of lipid III due to a defect in the structural gene for the Fuc4NAc transferase (rffT). Expand
Presence of rfe genes in Escherichia coli: their participation in biosynthesis of O antigen and enterobacterial common antigen
In transduction experiments it could be shown that the appearance of the rough and CA- phenotype was due to a defect in the ilv-linked rfe region, and in the E. coli strains tested the rfe genes are involved in the synthesis of both O antigen and CA. Expand
Influence of the supramolecular structure of free lipid A on its biological activity.
The three-dimensional supramolecular structures and the states of order of the acyl chains of lipid A from different Gram-negative species were investigated at 40 degrees C, high water content, and different [lipid A]/[Mg2+] molar ratios using synchrotron radiation X-ray diffraction and Fourier-transform infrared spectroscopy and a clear correlation between the supramolescular structure and previously determined biological activities was found. Expand
Distribution and phylogenetic significance of 27-hydroxy-octacosanoic acid in lipopolysaccharides from bacteria belonging to the alpha-2 subgroup of Proteobacteria.
The results indicate that the presence of n-2-hydroxylated long-chain fatty acids in LPSs might be used as a chemophylogenetical marker. Expand