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Purification and characterization of the lipase from Serratia marcescens Sr41 8000 responsible for asymmetric hydrolysis of 3-phenylglycidic acid esters
Abstract A new lipase which enantioselectively hydrolyzes (±)- trans -3-(4-methoxyphenyl)glycidic acid methyl ester [(±)-MPGM], a key intermediate in the synthesis of diltiazem hydrochloride, wasExpand
Production of optically active 3-phenylglycidic acid ester by the lipase from Serratia marcescens on a hollow-fiber membrane reactor
Abstract (±)- trans -3-(4-Methoxyphenyl)glycidic acid methyl ester [(±)-MPGM], a key intermediate in the synthesis of diltiazem hydrochloride, was efficiently hydrolyzed by lipase from SerratiaExpand
Specific removal of endotoxin from protein solutions by immobilized histidine
A method for reducing endotoxin contamination in various solutions by immobilized histidine is described. Immobilized histidine is a porous adsorbent suitable for the adsorption of endotoxin with aExpand
Utilization of ATP-binding cassette exporter for hyperproduction of an exoprotein: construction of lipase-hyperproducing recombinant strains of Serratia marcescens
Abstract. The Serratia marcescens extracellular lipase (LipA) is an enzyme applicable to enantioselective hydrolysis of racemic substrates. The enzyme is secreted through an ATP-binding cassetteExpand
Lipase-catalyzed asymmetric hydrolysis of 3-phenylglycidic acid ester, the key intermediate in the synthesis of diltiazem hydrochloride
Abstract Asymmetric hydrolytic enzymes for trans -3-(4-methoxyphenyl)glycidic acid methyl ester [(±)-MPGM], a key intermediate in the synthesis of diltiazem hydrochloride that is useful as a coronaryExpand
Enzymatic resolution of diltiazem intermediate by Serratia marcescens lipase : molecular mechanism of lipase secretion and its industrial application
Abstract A lipase from Serratia marcescens was selected as an asymmetric hydrolytic enzyme for trans-3-(4-methoxyphenyl)glycidic acid methyl ester [(±)-MPGM], a key intermediate in the synthesis ofExpand
Microbial asymmetric reduction of (RS)-2-(4-methoxyphenyl)-1,5-benzothiazepin-3,4(2H,5H)-dione, the key intermediate in the synthesis of diltiazem hydrochloride
Abstract Microbial production of (2 S ,3 S )-2,3-dihydro-3-hydroxy-2-(4-methoxyphenyl)-1,5-benzothiazepin-4(5 H )-one [(2 S ,3 S )-2], a key intermediate in the synthesis of diltiazem hydrochloride,Expand
Isolation and analysis of lipase-overproducing mutants of Serratia marcescens.
We have isolated a lipase-overproducing mutant, GE14, from Serratia marcescens 8000 after three rounds of N-methyl-N'-nitro-N-nitrosoguanidine mutagenesis. The mutant GE14 produced 95 kU/ml ofExpand
Scalable Synthesis of a Nonracemic α-Arylpropionic Acid via Ketene Desymmetrization for a Glucokinase Activator
Process research and development for a synthesis of the chiral carboxylic acid (R)-2 as a key intermediate of the glucokinase activator (R)-1 is described. The construction of the stereocenter at theExpand
Preparation of (2S,3S)-2,3-dihydro-3-hydroxy-2-(4-methoxyphenyl)-1,5-benzothiazepin-4(5H)-one by microbial conversion
Abstract Microbial production of (2 S ,3 S )-2,3-dihydro-3-hydroxy-2-(4-methoxyphenyl)-1,5-benzothiazepin-4(5 H )-one [(2 S ,3 S )-DHMB], a key intermediate in the synthesis of diltiazemExpand
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