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PURIFICATION AND CHARACTERIZATION OF A CELLULASE FROM BACILLUS SUBTILIS YJ1
Broth incubated with Bacillus subtilis YJ1 at 37°C for 36 h was collected and removed bacterial cells by passing through a 0.45 µm membrane. A cellulase was purified to electrophoretical homogeneityExpand
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Early Initiation of Continuous Ambulatory Peritoneal Dialysis in Patients Undergoing Surgical Implantation of Tenckhoff Catheters
♦ Background: Nephrologists commonly recommend continuous ambulatory peritoneal dialysis (CAPD) with break-in periods of at least 2 weeks. We investigated the safety and feasibility of shorterExpand
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Expression and purification of pseudomonas aeruginosa keratinase in Bacillus subtilis DB104 expression system.
The DNA encoding Pseudomonas aeruginosa keratinase was ligated into pRPA expression vector and transformed into Bacillus subtilis DB104. Recombinant keratinase (rK), secreted by B. subtilis after 72Expand
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Cloning, expression, and purification of Pseudomonas aeruginosa keratinase in Escherichia coli AD494(DE3)pLysS expression system.
The DNA encoding keratinase from Pseudomonas aeruginosa was ligated into pET-43b(+) expression vector and transformed into Escherichia coli AD494(DE3)pLysS. After isopropylExpand
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Functional expression and characterization of keratinase from Pseudomonas aeruginosa in Pichia pastoris.
Recombinant keratinase (rK) from Pseudomonas aeruginosa was secreted by Pichia pastoris SMD1168H with a final yield of 580 mg/L (1.03 kU/mL) after 72 h of induction. The rK was purified after nickelExpand
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Reishi Polysaccharides Induce Immunoglobulin Production through the TLR4/TLR2-mediated Induction of Transcription Factor Blimp-1*
The polysaccharides of Ganoderma lucidum (Reishi) possess immunomodulation activities; however, their mode of molecular action in regulating each cellular subset in the immune system is still notExpand
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The role of polyphenol oxidase and peroxidase in the browning of water caltrop pericarp during heat treatment.
The mechanism of browning involving enzymatic browning was investigated in the pericarp of water caltrop, an Asian vegetable popular for its taste and medicinal properties. Polyphenol oxidase (PPO)Expand
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Anatomical and transcriptional dynamics of maize embryonic leaves during seed germination
Our anatomical analysis revealed that a dry maize seed contains four to five embryonic leaves at different developmental stages. Rudimentary kranz structure (KS) is apparent in the first leaf with aExpand
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A Novel puf-A Gene Predicted from Evolutionary Analysis Is Involved in the Development of Eyes and Primordial Germ-Cells
Although the human genome project has been completed for some time, the issue of the number of transcribed genes with identifiable biological functions remains unresolved. We used zebrafish as aExpand
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Transgenic rice plants expressing the ferredoxin-like protein (AP1) from sweet pepper show enhanced resistance to Xanthomonas oryzae pv. oryzae.
  • K. Tang, X. Sun, +6 authors T. Feng
  • Medicine, Biology
  • Plant science : an international journal of…
  • 1 April 2001
We used particle bombardment to cotransform mature seed-derived rice callus (Oryza sativa L., ssp. japonica, cv. Eyi 105) with plasmids containing the linked marker genes gusA and hpt, and the ap1Expand
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