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Investigation of sheep-associated malignant catarrhal fever virus infection in ruminants by PCR and competitive inhibition enzyme-linked immunosorbent assay
- H. Li, D. Shen, D. O'Toole, D. Knowles, J. Gorham, T. Crawford
- Medicine, BiologyJournal of clinical microbiology
- 1 August 1995
Although all colostrum and milk samples from infected ewes were strongly PCR positive, the appearance of detectable SA-MCFV DNA in lambs was correlated generally with antibody patterns, which suggests that the natural infection event in sheep may not occur during the perinatal period but occurs sometime later in life.
Recognition of another member of the malignant catarrhal fever virus group: an endemic gammaherpesvirus in domestic goats.
Combined serological and PCR-survey data demonstrated that this virus is endemic in goats and its transmission pattern may be similar to that of ovine herpesvirus-2 in sheep.
Chronic and recovered cases of sheep-associated malignant catarrhal fever in cattle
Observations indicate that recovery and chronic disease are a significant part of the clinical spectrum of MCF and that such cases occur with some frequency in the area studied.
The Pathology of Malignant Catarrhal Fever, With an Emphasis on Ovine Herpesvirus 2
This review focuses on recent efforts to understand the pathogenesis of MCF, particularly the sheep-associated form of the disease, with the goal of developing rational control methods, including vaccination.
Competitive inhibition enzyme-linked immunosorbent assay for antibody in sheep and other ruminants to a conserved epitope of malignant catarrhal fever virus
- H. Li, D. Shen, D. Knowles, J. Gorham, T. Crawford
- BiologyJournal of clinical microbiology
- 1 July 1994
An attempt was made to identify an epitope conserved among all isolates of MCF viruses, by using a monoclonal antibody produced against a previously reported U.S. isolate of MCFs, and a competitive inhibition enzyme-linked immunosorbent assay (ELISA) based on MAb 15-A was developed.
Field Validation of Laboratory Tests for Clinical Diagnosis of Sheep-Associated Malignant Catarrhal Fever
- U. Müller-Doblies, H. Li, B. Hauser, H. Adler, M. Ackermann
- Biology, MedicineJournal of Clinical Microbiology
- 1 October 1998
Using clinically diagnosed field cases, a seminested PCR and a competitive inhibition enzyme-linked immunosorbent assay (CI-ELISA) are evaluated and compared in the diagnosis of SA-MCF in cattle with histopathology as a provisional “gold standard".
Malignant Catarrhal Fever in a Bison (Bison Bison) Feedlot, 1993–2000
- D. O'Toole, H. Li, C. Sourk, D. Montgomery, T. Crawford
- MedicineJournal of veterinary diagnostic investigation…
- 1 May 2002
A fatal enteric syndrome was identified in American bison (Bison bison) at a large feedlot in the American Midwest in early 1998, and a prospective study was undertaken to establish the importance of MCF relative to other fatal diseases at the feedlot.
Levels of ovine herpesvirus 2 DNA in nasal secretions and blood of sheep: implications for transmission.
Identification and characterization of the major proteins of malignant catarrhal fever virus.
- H. Li, D. Shen, W. Davis, D. Knowles, J. Gorham, T. Crawford
- BiologyThe Journal of general virology
Comparison of three MCFV isolates revealed no apparent differences in immunoprecipitation patterns with the single exception that the 110 kDa protein of WC-11 was slightly smaller than its counterpart in the Minnesota isolate.