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Colorimetric determination of cell numbers by Janus green staining.
Working under standardized conditions, Janus green uptake in several cell lines is shown to be linearly correlated with cell numbers over a broad range of cell densities, in MC3T3-E1 cells from about 3% up to more than 300% of confluency.
Quantitative analysis of memantine in human plasma by gas chromatography/negative ion chemical ionization/mass spectrometry.
The method is rugged, rapid and robust and was applied to the batch determination of memantine during pharmacokinetic profiling of the drug.
Plasma and interstitial glucose dynamics after intravenous glucose injection: evaluation of the single-compartment glucose distribution assumption in the minimal models.
The results suggest that the Hot-MM is more appropriate for the evaluation of glucose effectiveness than the Cold-MM, and the requirements for a single-compartment representation of glucose kinetics are not satisfied during at least the first 22 min of an IVGTT.
Rapid analysis of non-esterified fatty acids as methyl esters from different biological specimens by gas chromatography after one-step esterification.
A rapid gas chromatographic method for the determination of medium-chain and long-chain free fatty acids (C14:0 to C24:0 fatty acids) from different biological specimens is presented. After a rapid
On the enigmatic scent glands of dyspnoan harvestmen (Arachnida, Opiliones): first evidence for the production of volatile secretions
It is shown that at least certain nemastomatid Dyspnoi, namely all three species of genus Carinostoma, indeed produce a volatile secretion, comprising octan-3-one, 6-methyl-5-hepten-2-one and acetophenone in species-specific combinations.
Chrysomelidial in the Opisthonotal Glands of the Oribatid Mite, Oribotritia berlesei
The chrysomelidials, as well as β-springene and octadecadienal, are newly identified compounds in the opisthonotal glands of oribatid mites and have chemotaxonomic potential for this group.
Benzoquinone-rich exudates from the harvestman Pachylus paessleri (Opiliones: Gonyleptidae: Pachylinae)
While dimethyl- and trimethyl-benzoquinones are widespread in scent gland secretions of Gonyleptoidea, 2,5-dimethyl-3-ethyl-1,4- Benzoquinone and 2,ethyl-5-methyl-1-4-benzosquinone are reported for the first time in Opiliones.
Bone‐resorbing activity of thyroid hormones is related to prostaglandin production in cultured neonatal mouse calvaria
Thyroid hormone action on bone is also mediated by a PG‐independent mechanism, and in some experiments indomethacin reduced thyroid hormone‐mediated bone resorption by only 50%.
The regulatory role of thyroid hormones in bone cell growth and differentiation.
It is concluded that thyroid hormones accelerate osteoblastic differentiation and a novel role of triiodothyronine as inhibitor of growth factor induced transcriptional expression of regulatory genes (c-fos, c-jun) is suggested.
Phloretin ameliorates 2-chlorohexadecanal-mediated brain microvascular endothelial cell dysfunction in vitro
2-ClHDA has the potential to induce BBB breakdown under inflammatory conditions and that phloretin confers protection in this experimental setting, and potential protective effects of several natural polyphenols on in vitro BBB function are investigated.