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Isolation of an RNA-Directed RNA Polymerase–Specific cDNA Clone from Tomato
TLDR
Antibodies that were raised against synthetic peptides whose sequences have been deduced from the ORF were shown to specifically detect the 127-kD tomato RdRP protein.
Correlation between structure and pathogenicity of potato spindle tuber viroid (PSTV)
TLDR
A model for the mechanism of viroid pathogenicity implies that the nucleotides of the VM region specify and modulate the binding‐ and hence the competition‐potential of the PSTV RNA molecule for a still unknown host factor(s) and thus determine the virulence of PSTV.
Nucleotide sequence and secondary structure of citrus exocortis and chrysanthemum stunt viroid.
TLDR
It is postulate that the resulting 'secondary' hairpins, being formed and observed in vitro during the complex process of thermal denaturation of viroid RNA, must have a vital, although yet unknown, function in vivo.
A single nucleotide substitution converts potato spindle tuber viroid (PSTVd) from a noninfectious to an infectious RNA for nicotiana tabacum.
TLDR
The sequence analysis of PSTVd NT revealed a single nucleotide substitution from C-->U at position 259, which might be involved in protein binding and in viroid processing in both tobacco and tomato.
Nucleotide sequence and secondary structure of potato spindle tuber viroid
TLDR
The viroid of the potato spindle tuber disease is a covalently closed ring of 359 ribonucleotides that is the first pathogen of a eukaryotic organism for which the complete molecular structure has been established.
Heavy de novo methylation at symmetrical and non-symmetrical sites is a hallmark of RNA-directed DNA methylation.
TLDR
The observation that the heavy methylation was essentially co-extensive with the length of the PSTVd cDNA sequences provided evidence that a direct RNA-DNA interaction can act as a strong and highly specific signal for de novo DNA methylation.
Secondary structure probing of potato spindle tuber viroid (PSTVd) and sequence comparison with other small pathogenic RNA replicons provides evidence for central non-canonical base-pairs, large
TLDR
Non-denaturing gel electrophoresis shows that linear (non-circular) unit-length (+)strand RNA molecules of a lethal PSTVd variant were produced which are able to initiate typical disease symptoms when inoculated into tomato.
Conformational transitions in viroids and virusoids: comparison of results from energy minimization algorithm and from experimental data.
TLDR
For viroids the calculations confirm earlier theoretical and experimental results about the extended native structure and the highly cooperative transition into a branched structure and confirms the uniqueness of viroid structure.
DNA-dependent RNA polymerase II of plant origin transcribes viroid RNA into full-length copies
TLDR
Findings suggest that viroids replicate by an entirely novel mechanism in which infecting viroid RNA molecules are copied by the host enzyme which is normally responsible for the synthesis of nuclear precursors to messenger RNA.
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