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RNA-directed de novo methylation of genomic sequences in plants.

• Michael Wassenegger, S. Heimes, Leonhard Riedel, H. L. Sänger
• Cell
• 1994

One monomeric and three oligomeric potato spindle tuber viroid (PSTVd) cDNA units were introduced into the tobacco genome via the Agrobacterium-mediated leaf-disc transformation. Southern analysis of… (More)

Heavy de novo methylation at symmetrical and non-symmetrical sites is a hallmark of RNA-directed DNA methylation.

• Thierry Pélissier, S Thalmeir, Dr. André Kempe, H. L. Sänger, Michael Wassenegger
• Nucleic acids research
• 1999

Previous analysis of potato spindle tuber viroid (PSTVd) RNA-infected tobacco plants has suggested that an RNA-DNA interaction could trigger de novo methylation of PSTVd transgene sequences. Using… (More)

Isolation of an RNA-directed RNA polymerase-specific cDNA clone from tomato.

• Walter Schiebel, Thierry Pélissier, +6 authors Michael Wassenegger
• The Plant cell
• 1998

A 3600-bp RNA-directed RNA polymerase (RdRP)-specific cDNA comprising an open reading frame (ORF) of 1114 amino acids was isolated from tomato. The putative protein encoded by this ORF does not share… (More)

Correlation between structure and pathogenicity of potato spindle tuber viroid (PSTV).

• Martina Schnoelzer, B. Haas, K Raam, Hans Carlos Hofmann, H. L. Sänger
• The EMBO journal
• 1985

Sequence analysis by primer-extension at the level of their cDNA showed that the RNA genomes of various field isolates of potato spindle tuber viroid (PSTV) of different virulence differ from each… (More)

A single nucleotide substitution converts potato spindle tuber viroid (PSTVd) from a noninfectious to an infectious RNA for nicotiana tabacum.

• Michael Wassenegger, R. L. Spieker, S Thalmeir, Frank Gast, Leonhard Riedel, H. L. Sänger
• Virology
• 1996

Mechanical inoculation of Nicotiana tabacum with the PSTVd isolate KF 440-2 from the host plant tomato resulted in the de novo emergence, replication, and accumulation of a new "tobacco variant,"… (More)

Conformational transitions in viroids and virusoids: comparison of results from energy minimization algorithm and from experimental data.

• G. Steger, Hans Carlos Hofmann, +4 authors Detlev Riesner
• Journal of biomolecular structure & dynamics
• 1984

Viroids are single-stranded circular RNA molecules of 240 to 400 nucleotides which are pathogens of certain higher plants and replicate autonomously in the host cell. Virusoids are similar to viroids… (More)

Secondary structure probing of potato spindle tuber viroid (PSTVd) and sequence comparison with other small pathogenic RNA replicons provides evidence for central non-canonical base-pairs, large…

• Frank Gast, Dr. André Kempe, R. L. Spieker, H. L. Sänger
• Journal of molecular biology
• 1996

Using PCR and in vitro transcription, linear (non-circular) unit-length (+)strand RNA molecules of a lethal PSTVd variant were produced which are able to initiate typical disease symptoms when… (More)

Nucleotide sequence and secondary structure of citrus exocortis and chrysanthemum stunt viroid.

• Howard Gross, Guido Krupp, +6 authors H. L. Sänger
• European journal of biochemistry
• 1982

The complete nucleotide sequence of citrus exocortis viroid (CEV, propagated in Gymura) and chrysanthemum stunt viroid (CSV, propagated in Cineraria) has been established, using labelling in vitro… (More)

An infectious viroid RNA replicon evolved from an in vitro-generated non-infectious viroid deletion mutant via a complementary deletion in vivo.

• Michael Wassenegger, S. Heimes, H. L. Sänger
• The EMBO journal
• 1994

The 359 nucleotides (nt) long potato spindle tuber prototype viroid (PSTVd) is sensitive to experimentally introduced mutations as the substitution or deletion of a single nucleotide usually… (More)

Structure and structure formation of viroids.

• Detlev Riesner, K. Henco, +6 authors H. L. Sänger
• Journal of molecular biology
• 1979

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