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Tonicity-responsive enhancer binding protein, a rel-like protein that stimulates transcription in response to hypertonicity.
TLDR
CDNA cloning of human TonE binding protein (TonEBP) is reported, a transcription factor that stimulates transcription through its binding to TonE sequences via a Rel-like DNA binding domain that plays a key role in hypertonicity-induced transcriptional stimulation of SMIT, sodium/chloride/betaine cotransporter, and aldose reductase. Expand
Cloning of the cDNa for a Na+/myo-inositol cotransporter, a hypertonicity stress protein.
TLDR
Transporter mRNA is present in kidney and brain and is markedly induced in MDCK cells by medium hypertonicity, demonstrating that adaptation to hypertonic stress involves up-regulation of transporter mRNA accumulation. Expand
Cloning of a Na(+)- and Cl(-)-dependent betaine transporter that is regulated by hypertonicity.
TLDR
Expression in oocytes indicates that the renal BGT-1 protein has both betaine and GABA transport activities that are Cl(-)- as well as Na(+)-dependent and functionally similar to betaineand GABA transport in MDCK cells. Expand
Bidirectional regulation of tonicity-responsive enhancer binding protein in response to changes in tonicity.
TLDR
Exposure to hypotonic medium leads to a dramatic downregulation of TonEBP both in abundance and nuclear distribution, indicating that under isotonic conditions, TonE BP is at a low-level activated state and can respond to both increase and decrease in tonicity. Expand
Molecular cloning of the cDNA for an MDCK cell Na(+)- and Cl(-)-dependent taurine transporter that is regulated by hypertonicity.
TLDR
Northern hybridization indicates that mRNA for the taurine transporter is present in renal cortex and medulla, ileal mucosa, brain, liver, and heart, suggesting that regulation of transport activity by medium hypertonicity occurs at the level of mRNA accumulation. Expand
TonEBP/NFAT5 Stimulates Transcription of HSP70 in Response to Hypertonicity
TLDR
It is concluded that TonEBP is a master regulator of the renal medulla for cellular protection against high osmolality via organic osmolytes and molecular chaperones. Expand
The tonicity-sensitive element that mediates increased transcription of the betaine transporter gene in response to hypertonic stress.
TLDR
To investigate the molecular mechanism of transcriptional regulation by tonicity, the 5'-flanking region of the gene is characterized and a smaller region that is required for hypertonicity to induce increased expression and a slowly migrating band on mobility shift assays is identified. Expand
Transcription of the Sodium/myo-Inositol Cotransporter Gene Is Regulated by Multiple Tonicity-responsive Enhancers Spread over 50 Kilobase Pairs in the 5′-Flanking Region*
TLDR
In vivo methylation experiments demonstrated that exposure of cells to hypertonicity increases the binding of tonicity-responsive enhancer binding protein to the enhancer sites, indicating that all of these enhancers are involved in the transcriptional stimulation. Expand
Molecular cloning of the cDNA for an MDCK cell Na(+)- and Cl(-)-dependent taurine transporter that is regulated by hypertonicity.
Abstract Cells in the hypertonic renal medulla maintain their intracellular ion concentration at isotonic levels, despite much higher concentrations of extracellular electrolytes, by accumulatingExpand
Cell volume regulated transporters of compatible osmolytes.
TLDR
When cells return to an iso-osmotic environment, osmolytes are rapidly lost through a pathway that current evidence indicates may be a volume-sensitive chloride channel. Expand
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