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Molecular Characterization of the Fecal Microbiota in Patients with Nonalcoholic Steatohepatitis – A Longitudinal Study
TLDR
NASH patients have fecal dysbiosis, and changes in microbiota correlate with improvement in hepatic steatosis, and bacterial biodiversity did not differ between NASH patients and controls, and did not change with probiotic treatment. Expand
BSRD: a repository for bacterial small regulatory RNA
TLDR
BSRD is developed, a comprehensive bacterial sRNAs database, as a repository for published bacterial sRNA sequences with annotations and expression profiles, and a novel RNA-Seq analysis platform, sRNADeep, to characterize s RNAs in large-scale transcriptome sequencing projects. Expand
Composition and genetic diversity of picoeukaryotes in subtropical coastal waters as revealed by 454 pyrosequencing
TLDR
Diversity of picoeukaryotes was higher in an oligomesotrophic bay than in a eutrophic bay, and the total diversity was underestimated at a genetic difference of ⩽2%. Expand
DNA methods for identification of Chinese medicinal materials
TLDR
This review provides an informative introduction to DNA methods for authentication of Chinese medicinal materials based on sequencing, hybridization and polymerase chain reaction (PCR) and their suitability for different identification objectives is discussed. Expand
Cloning, expression, and primary structure of Metapenaeus ensis tropomyosin, the major heat-stable shrimp allergen.
TLDR
The primary structure of the major heat-stable allergen of shrimp is determined by cloning, expression, nucleotide sequencing, and amino acid sequence determination of an IgE-reactive cDNA clone, Met e I, isolated from a Metapenaeus ensis expression library in lambda gt 11. Expand
Identification and molecular characterization of Charybdis feriatus tropomyosin, the major crab allergen.
TLDR
This 34-kd protein, designated as Cha f 1, is the first identified major allergen of crab and nucleotide and amino acid comparison shows that this protein is the crab tropomyosin. Expand
IgE reactivity against a cross-reactive allergen in crustacea and mollusca: evidence for tropomyosin as the common allergen.
TLDR
The allergic epitopes on tropomyosin are conserved among invertebrates including not only shellfish but also insects, suggesting that persons sensitive to shrimp should undergo further study for potential cross-reactive inhalant or ingested insect sensitivity. Expand
The genetic structure of the A mating-type locus of Lentinula edodes.
TLDR
This work identified the A mating-type locus from the first draft genome sequence of L. edodes and characterized multiple alleles from different monokaryotic strains, the first known exception among Homobasidiomycetes, in which the mitochondrial intermediate peptidase (mip) gene is not closely linked to A locus. Expand
Sputum Microbiota in Tuberculosis as Revealed by 16S rRNA Pyrosequencing
TLDR
The composition and diversity of sputum microbiota in TB infection was characterized for the first time by using high-throughput pyrosequencing and lays the framework for examination of potential roles played by the diverse microbiota inTB pathogenesis and progression, and could ultimately facilitate advances in TB treatment. Expand
Rapid Change of Microbiota Diversity in the Gut but Not the Hepatopancreas During Gonadal Development of the New Shrimp Model Neocaridina denticulata
TLDR
This study provides the first evidence that microbiota modified differentially in specific regions of the digestive tract during gonadal development of crustaceans. Expand
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