The bearing of these findings on various problems, including the number of NAD(+)-NADH pools in liver cells; the applicability of the method to tissues other than liver; the transhydrogenase activity of glutamate dehydrogenase; the physiological significance of the difference of the redox states of mitochondria and cytoplasm; aspects of the regulation of theredox state of cell compartments; the steady-state concentration of mitochondrial oxaloacetate.Expand
Agreement between two highly active enzyme systems in the same compartment is taken as evidence of the existence of near-equilibrium in both these systems and suggests that free cytosolic [sigma ADP] is probably 20-fold lower than measured cell ADP content in mitochondrial-containing tissues.Expand
The application of the method of calculation to data published by Kraupp, Adler-Kastner, Niessner & Plank (1967), Goldberg, Passonneau & Lowry (1966) and Kauffman, Brown,passonneau and Lowry (1968) shows that the redox states of the NAD and NADP couples in cardiac-muscle cytoplasm and in mouse-brain cytop lasm are of the same order as those in rat liver.Expand
There is a relationship between intracellular pH, phosphofructokinase inhibition and CO2 production, suggesting that the antifungal action of Benzoate is caused by an accumulation of benzoate at low external pH, which lowers the intrace cellular pH into the range where phosphofructureokinase is sensitive.Expand
This address by Sir Hans Krebs at the inauguration of the Department of Biochemistry at the University of Newcastle upon Tyne earlier this year argued for the perpetuation of centres of excellence.Expand
The results indicate that ketone bodies are major metabolic fuels of the brain of the suckling rat under normal conditions and are independent of the nutritional state.Expand
The hypothesis that GSSG exerts a fine control of the pentose phosphate cycle by counteracting the NADPH inhibition of glucose 6-phosphate dehydrogenase is discussed.Expand
The absolute level of Q O 2 in the new media (which apart from glucose contain pyruvate, fumarate and L-glutamate) was higher than the values reported in the literature for saline media and of the same order as the highest values recorded for serum.Expand
This investigation found that glutamic acid behaves differently from all the other ax-amino-acids in guinea-pig and rabbit kidneys, in that, although it increases the oxygen uptake even more than any other amino-acid of the i-series, in most cases it actually diminishes ammonia formation.Expand
The enol generated in the first step is converted into a nucleophile by the abstraction of the enol hydrogen by His274 now functioning as a general base. The electrons of the double bond attack the… Expand