• Publications
  • Influence
Biochemical characterization of NfsA, the Escherichia coli major nitroreductase exhibiting a high amino acid sequence homology to Frp, a Vibrio harveyi flavin oxidoreductase
TLDR
Although no appreciable sequence similarity was detected between two E. coli nitroreductases, NfsA exhibited a low level of the flavin reductase activity and a broad electron acceptor specificity similar to those of NfsB, it was suggested that NFSA is a nonglobular protein with a molecular weight of 26,799 and is associated tightly with a flavin mononucleotide. Expand
Gene cloning, purification, and characterization of NfsB, a minor oxygen-insensitive nitroreductase from Escherichia coli, similar in biochemical properties to FRase I, the major flavin reductase in
TLDR
It was shown that NfsB has a broad electron acceptor specificity and is associated with a low level of the NAD(P)H-flavin oxidoreductase, and could serve as an effective substitute for FMN. Expand
A structural code for discriminating between transcription signals revealed by the feast/famine regulatory protein DM1 in complex with ligands.
TLDR
By analyzing residues surrounding the ligand side chain, partner ligands were identified for various FFRPs from Pyrococcus, e.g., lysine facilitates homo-Octamerization of FL11, and arginine facilitates hetero-octamerizationof FL11 and DM1. Expand
Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.
TLDR
The complete genomic sequence of the archaeon Thermoplasma volcanium, possessing optimum growth temperature (OGT) of 60 degrees C, is reported and a number of strong correlations have been identified between characteristics of genomic organization and the OGT. Expand
Feast/famine regulatory proteins (FFRPs): Escherichia coli Lrp, AsnC and related archaeal transcription factors.
TLDR
The genome-wide regulation by feast/famine regulatory proteins is traceable back to their common ancestor, being the prototype of highly differentiated transcription regulatory mechanisms found in organisms nowadays. Expand
Feast/famine regulation by transcription factor FL11 for the survival of the hyperthermophilic archaeon Pyrococcus OT3.
TLDR
Dimmer-binding sites were identified in promoters of approximately 200 transcription units coding, for example, H+-ATPase and NAD(P)H dehydrogenase, and this regulation resembles global regulation by Escherichia coli leucine-responsive regulatory protein, and hints at a prototype of transcription regulations now highly diverged. Expand
The archaeal feast/famine regulatory protein: potential roles of its assembly forms for regulating transcription.
TLDR
This paper describes two forms of the archaeal FFRP FL11 (pot0434017), both assembled from dimers, and proposes a possible mechanism for regulating a number of genes by varying assembly forms and by combining different FFRPs into these assemblies, responding to environmental changes. Expand
Increased production of fatty acids and triglycerides in Aspergillus oryzae by enhancing expressions of fatty acid synthesis-related genes
TLDR
By simply increasing the expression of the fatty acid synthase genes, this work successfully increased the production of fatty acids and triglycerides by more than two-fold, demonstrating that a simple increase in the abundance of fatty acid synthesis-related genes can increase the detectable amount of fat. Expand
Genome Sequence of the Basidiomycetous Yeast Pseudozyma antarctica T-34, a Producer of the Glycolipid Biosurfactants Mannosylerythritol Lipids
TLDR
The genome sequence of Pseudozyma antarctica T-34 was determined and annotated to provide insights into the properties of this yeast that make it superior for use in the production of functional glycolipids, leading to the further development of P. antarctic for industrial applications. Expand
Identification and characterization of genes responsible for biosynthesis of kojic acid, an industrially important compound from Aspergillus oryzae.
TLDR
Two genes of unknown function were found to be responsible for kojic acid biosynthesis, one having an oxidoreductase motif and the other a transporter motif, closely associated in the genome, showing typical characteristics of genes involved in secondary metabolism. Expand
...
1
2
3
4
5
...