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Development of alternative plant vitrification solutions in droplet-vitrification procedures.
Alternative vitrification solutions modified from the original PVS2 vitrification solution by increasing glycerol and sucrose and/or decreasing dimethylsulfoxide and ethylene glycol concentration can be applied when explants are of medium size, tolerant to chemical toxicity and moderately sensitive to osmotic stress.
Importance of explant size and origin and of preconditioning treatments for cryopreservation of garlic shoot apices by vitrification.
This paper investigates the effect of the origin and size of the explants employed and of the preconditioning and loading treatments on survival and regeneration of cryopreserved garlic shoot apices using vitrification with the PVS3 vitrification solution.
Cryopreservation of Citrus madurensis embryonic axes by vitrification: importance of loading and treatment with vitrification solution.
A three-step vitrification protocol, involving the treatment of embryonic axes at 0 degree C with half strength PVS2 solution for 20 min then with full strength P VS2 for an additional 40 min was more efficient than a two-step protocol that involved treatment of axes directly with fullstrength PVS1 solution for 60 min.
Isolation and characteristics of eight novel polymorphic microsatellite loci from the genome of garlic (Allium sativum L.)
Eight novel polymorphic SSR markers, developed from an enriched genomic library of garlic, are characterized and should prove very useful tools for genotypes identification, assessment of genetic diversity and population structure in garlic.
Cross-amplification of SSR markers developed from Allium sativum to other Allium species
For genetic analysis of the genus Allium, which is composed of diverse species, 50 transferable and polymorphic microsatellite markers from A. sativum were acquired and tested for transferability in five Allium species and the dinucleotide motif was the most prevalent.
Cryopreservation of Citrus aurantifolia seeds and embryonic axes using a desiccation protocol.
The desiccation and freezing tolerance of seeds, with and without testas, and embryonic axes of Citrus aurantifolia were investigated and survival before and after cryopreservation was higher for seeds without testa and non-desiccated embryonic axes.
Cryopreservation of tea (Camellia sinensis L.) seeds and embryonic axes.
The maturity stage of the seeds from which CEAs were extracted had an important effect on their survival and plant production percentages, mature seeds providing better results than early mature and late mature seeds.
MYB Transcription Factors Regulate Glucosinolate Biosynthesis in Different Organs of Chinese Cabbage (Brassica rapa ssp. pekinensis)
The investigation of the expression of seven MYB transcription factors involved in aliphatic and indolic glucosinolate (GSL) biosynthesis and the analysis of their content in different organs of Chinese cabbage may be helpful to understand the mechanism of MYB TFs regulating GSL biosynthesis in Chinese cabbage.
Cryopreservation of cultivated and wild potato varieties by droplet vitrification: effect of subculture of mother-plants and of preculture of shoot tips.
The results indicate that the parameters of the subculture of mother-plants and of preculture of shoot tips should be carefully optimized, especially in the case of wild species.
Ethylene Inhibitors Enhance Shoot Organogenesis of Gloxinia (Sinningia speciosa)
Shoot organogenesis and plant regeneration in Sinningia speciosa were improved using ethylene inhibitors significantly improved the regeneration frequency giving higher shoots per explant and longer shoot length.