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Molecular cloning of a novel mammalian calcium-dependent protease distinct from both m- and mu-types. Specific expression of the mRNA in skeletal muscle.
Northern blot analysis revealed that the mRNA for p94 exists only in skeletal muscle with none detected in other tissues including heart muscle and smooth muscles such as intestine, in contrast to the ubiquitous expression of mu- and m-types. Expand
A new NEDD8-ligating system for cullin-4A.
A novel ubiquitinylation-related pathway for modification by NEDD8 was reported, and the major target protein modified was found to be Hs-cullin- 4A (Cul-4A), a member of the family of human cullin/Cdc53 proteins functioning as an essential component of a multifunctional Ub-protein ligase E3 complex that has a critical role in Ub-mediated proteolysis. Expand
Classification and evolution of EF-hand proteins
Forty-five distinct subfamilies of EF-hand proteins have been identified; many sequence and chem-ical characteristics do not show systemic trends by subfamily or species of host organisms; such homoplasy is widespread. Expand
Purification of native p94, a muscle-specific calpain, and characterization of its autolysis.
The autolysis sites in p94 clearly showed a different specificity relative to the autolytic and proteolytic cleavage sites of the ubiquitous mu- and m-calpains, in its preference for residues at the P3 to P1' sites, indicating a distinct substrate specificity and function for the muscle enzyme. Expand
Calcium‐activated neutral protease and its endogenous inhibitor Activation at the cell membrane and biological function
The structures of calcium‐activated neutral protease (CANP) and its endogenous inhibitor elucidated recently have revealed novel features with respect to their structure‐function relationship andExpand
Molecular structure of a gene, VMA1, encoding the catalytic subunit of H(+)-translocating adenosine triphosphatase from vacuolar membranes of Saccharomyces cerevisiae.
Results suggest that a novel processing mechanism, which might involve a post-translational excision of the internal region followed by peptide ligation, operates on the yeast VMA1 product, and suggest that the vacuolar membrane H(+)-ATPase participates in maintenance of cytoplasmic Ca2+ homeostasis. Expand
Purification and characterization of a Z-Leu-Leu-Leu-MCA degrading protease expected to regulate neurite formation: a novel catalytic activity in proteasome.
The activity of the proteasome was inhibited efficiently by ZLLLa1, and was different from known catalytic activities of proteasomes in some aspects, suggesting it to be a novel one, and the proteAsome may be involved in the regulation of neurite formation in PC12 cells. Expand
Purification and properties of two type-B alpha-L-arabinofuranosidases produced by Penicillium chrysogenum.
Two distinct extracellular alpha-L-arabinofuranosidases (AFases; EC 3.55) were purified from the culture filtrate of Penicillium chrysogenum 31B and displayed broad substrate specificities; they released arabinose from branched arabinan, debranchedArabinan), arabinoxylan, arabinogalactan, and arabino-oligosaccharides. Expand
Xenopus M phase MAP kinase: isolation of its cDNA and activation by MPF.
It is suggested that MAP kinase functions as an intermediate between MPF and the interphase‐M phase transition of microtubule organization and may be regulated posttranslationally, most likely by phosphorylation. Expand
Two distinct cDNAs for human IMP dehydrogenase.
This is the first report suggesting the existence of two distinct types of human IMP dehydrogenase molecular species which may have different sensitivities to the drugs targeted against IMP dehydrogensase. Expand