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Chitosan-elicited callose synthesis in soybean cells as a ca-dependent process.
It is concluded that this Ca( 2+)-dependent enzyme is directly activated by the influx of Ca(2+) occurring concomitantly with the leakage of cell constituents. Expand
Effect of Chitosan on Membrane Permeability of Suspension-Cultured Glycine max and Phaseolus vulgaris Cells.
Treatment of suspension-cultured Glycine max cv Harosoy 63 cells with soluble chitosan increased membrane permeability as shown by leakage of electrolytes, protein, and UV absorbing material, and polycation-induced leakage was inhibited by divalent cations. Expand
The degrees of polymerization and N-acetylation of chitosan determine its ability to elicit callose formation in suspension cells and protoplasts of Catharanthus roseus
Results indicate that the primary interaction involved the amino groups of chitosan and numerous negative charges at the surface of the plasma membrane with spacing in the nanometer range and occurring regularly over micrometer stretches, and Charged phospholipid head-groups may fulfill these requirements. Expand
Release of Calcium from Suspension-Cultured Glycine max Cells by Chitosan, Other Polycations, and Polyamines in Relation to Effects on Membrane Permeability.
The results suggest that the effect of polycations on permeability is not the direct result of calcium displacement from the cell surface but is probably due to cross-linking of surface components. Expand
Silica deposition by a strongly cationic proline-rich protein from systemically resistant cucumber plants.
It is suggested that during induction of SAR, the synthesis of a strongly cationic PRP prepares the cell walls for enhanced silica deposition which is known to participate in cell wall reinforcement, localized at the site of attempted penetration of fungi into epidermal cells. Expand
Characterization, enzymatic and lectin properties of isolated membranes from Phaseolus aureus.
In vivo incorporation of D-[U-14C]glucose and subsequent isolation and solubilization of the different membranes showed that sugar-containing polymers could be released without hydrolytic techniques and were present in the equivalent extracts that exhibited lectin activity. Expand
Competence for Elicitation of H2O2 in Hypocotyls of Cucumber Is Induced by Breaching the Cuticle and Is Enhanced by Salicylic Acid
The results suggest that the cucumber hypocotyl epidermis becomes conditioned for competence to produce H2O2 in response to elicitors by a stimulus resulting from breaching the cuticle and/or cutting segments. Expand
Fluorometric Measurement of Callose and Other 1,3-β-Glucans
The term callose originates from cytochemical observations. Over the last hundred years many examples have been reported where staining and fluorescence techniques reveal that certain cell-wallExpand
Pretreatment of Parsley Suspension Cultures with Salicylic Acid Enhances Spontaneous and Elicited Production of H2O2
The conditioning of the parsley suspension culture by SA represents a model for the long-term regulation of apoplastic H2O2 concentration by this signal substance, as suggested previously for the wound hormone methyl jasmonate. Expand