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ITS as an environmental DNA barcode for fungi: an in silico approach reveals potential PCR biases
BackgroundDuring the last 15 years the internal transcribed spacer (ITS) of nuclear DNA has been used as a target for analyzing fungal diversity in environmental samples, and has recently beenExpand
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Fungal community analysis by high-throughput sequencing of amplified markers – a user's guide
Novel high-throughput sequencing methods outperform earlier approaches in terms of resolution and magnitude. They enable identification and relative quantification of community members and offer newExpand
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ITS1 versus ITS2 as DNA metabarcodes for fungi
The nuclear ribosomal Internal Transcribed Spacer ITS region is widely used as a DNA metabarcoding marker to characterize the diversity and composition of fungal communities. In ampliconExpand
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Mushroom fruiting and climate change
Many species of fungi produce ephemeral autumnal fruiting bodies to spread and multiply. Despite their attraction for mushroom pickers and their economic importance, little is known about theExpand
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Changes in the root‐associated fungal communities along a primary succession gradient analysed by 454 pyrosequencing
We investigated changes in the root‐associated fungal communities associated with the ectomycorrhizal herb Bistorta vivipara along a primary succession gradient using 454 amplicon sequencing. OurExpand
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New environmental metabarcodes for analysing soil DNA: potential for studying past and present ecosystems
Metabarcoding approaches use total and typically degraded DNA from environmental samples to analyse biotic assemblages and can potentially be carried out for any kinds of organisms in an ecosystem.Expand
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Amplicon‐pyrosequencing‐based detection of compositional shifts in bryophyte‐associated fungal communities along an elevation gradient
Although bryophytes are a dominant vegetation component of boreal and alpine ecosystems, little is known about their associated fungal communities. HPLC assays of ergosterol (fungal biomass) andExpand
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Seasonal trends in the biomass and structure of bryophyte-associated fungal communities explored by 454 pyrosequencing.
Bryophytes are a dominant vegetation component of the boreal forest, but little is known about their associated fungal communities, including seasonal variation within them. Seasonal variation in theExpand
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Fungi ahoy! Diversity on marine wooden substrata in the high North
Marine fungi are severely understudied in the polar regions. We used molecularly identi- fied cultures to study fungi inhabiting 50 intertidal and sea-floor logs along the North Norwegian coast. TheExpand
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Accelerated nrDNA evolution and profound AT bias in the medicinal fungus Cordyceps sinensis.
Cordyceps sinensis is a reputed medicinal fungus growing parasitically on buried larvae of ghost moths in Asian high-altitude grassland ecosystems. We have analysed the intraspecific ITS nrDNA (ITS1,Expand
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