H. Kasai

Publications138
h-index35
Citations4,226
Highly Influential Citations345
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Phylogeny of the genus Pseudomonas: intrageneric structure reconstructed from the nucleotide sequences of gyrB and rpoD genes.
Phylogenetic analysis of the genus Pseudomonas: was conducted by using the combined gyrB and rpoD nucleotide sequences of 31 validly described species of Pseudomonas: (a total of 125 strains).…
Intrageneric relationships among Micromonospora species deduced from gyrB-based phylogeny and DNA relatedness.
TLDR
The gyrB-based classification of genus Micromonospora agrees with the results of the DNA-DNA hybridization studies, indicating that this classification method is useful for analysing the phylogenetic relationships of high G+C Gram-positive bacteria at the level of the genomic species.
Differentiation of Phylogenetically Related Slowly Growing Mycobacteria by Their gyrB Sequences
TLDR
PCR and PCR-restriction fragment length polymorphism methods are developed to discriminate these species based on the differences found in the gyrB sequences.
Frondibacter mangrovi sp. nov., a member of the family Flavobacteriaceae isolated from seawater by in situ cultivation, and emended description of Frondibacter aureus.
A Gram-stain-negative, rod-shaped, non-motile, strictly aerobic, chemoheterotrophic, light-yellow-pigmented bacterium, designated strain 02OK1/10-76T, was isolated from a mangrove estuary in Japan by…
Molecular characterization of long direct repeat (LDR) sequences expressing a stable mRNA encoding for a 35‐amino‐acid cell‐killing peptide and a cis‐encoded small antisense RNA in Escherichia coli
TLDR
It is demonstrated that LDR‐D encodes a 35‐amino‐acid peptide, LdrD, the overexpression of which causes rapid cell killing and nucleoid condensation of the host cell, and it is proposed that lDR encode a toxin–antitoxin module.
Ferrimonas marina sp. nov.
TLDR
A novel Ferrimonas species is described on the basis of phenotypic, chemotaxonomic and phylogenetic studies, and it is proposed that the new isolates represent a novel species with the name Ferrim onas marina sp.
Mechercharimyces mesophilus gen. nov., sp. nov. and Mechercharimyces asporophorigenens sp. nov., antitumour substance-producing marine bacteria, and description of Thermoactinomycetaceae fam. nov.
TLDR
Two new isolates of Gram-positive, heterotrophic mesophiles isolated from marine lakes in the Republic of Palau are proposed, comprising two species based on genotypic and phenotypic criteria, including comparative gyrB and DNA-DNA relatedness data.
Alicyclobacillus pomorum sp. nov., a novel thermo-acidophilic, endospore-forming bacterium that does not possess omega-alicyclic fatty acids, and emended description of the genus Alicyclobacillus.
TLDR
Phylogenetic analyses based on both 16S rRNA and gyrB (DNA gyrase B subunit gene) gene sequences showed that strain 3A(T) falls into the Alicyclobacillus cluster, validated by significant bootstrap values and DNA-DNA hybridization values were under 13 %, indicating that strain3A (T) represents a distinct species.
Ruegeria mobilis sp. nov., a member of the Alphaproteobacteria isolated in Japan and Palau.
TLDR
The taxonomic positions of two bacterial strains isolated from marine environments of Japan and Palau, respectively, were determined by using a polyphasic approach and were considered to represent a novel species of the genus Ruegeria, for which the name Ruegersia mobilis sp.
Comparison of gyrB gene sequences, 16S rRNA gene sequences and DNA-DNA hybridization in the Bacillus subtilis group.
TLDR
It is concluded that the gyrB gene may be an efficient alternative target for identification and taxonomic analysis of members of the B. subtilis group and provide higher resolution than 16S rRNA gene sequences.
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