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Identification of natural rubber and characterization of rubber biosynthetic activity in fig tree.
The effect of EDTA and Mg(2+) ion on in vitro rubber biosynthesis in fig tree and rubber tree suggested that divalent metal ion present in the latex serum is an important factor in determining the different rubber biosynthetic activities in Fig tree and Rubber tree.
Construction of a bifunctional mRNA in the mouse by using the internal ribosomal entry site of the encephalomyocarditis virus.
Results indicate that dicistronic mRNAs are produced from the stably integrated vector in those ES clones and that both of the cistrons are translated to produce functional proteins.
A characteristic bent conformation of RNA pseudoknots promotes -1 frameshifting during translation of retroviral RNA.
- X. Chen, H. Kang, L. X. Shen, M. Chamorro, H. Varmus, I. Tinoco
- Biology, MedicineJournal of molecular biology
- 26 July 1996
The structures of two new RNA pseudoknots that provide one of the signals required for ribosomal frameshifting in mouse mammary tumor virus are determined by NMR, and the structures of all four pseudok nots are related to their frameshifted abilities.
Mutational analysis of the RNA pseudoknot involved in efficient ribosomal frameshifting in simian retrovirus-1.
The results reveal that coaxial stacking of stem 1 and stem 2 with a Watson-Crick A.U base pair in between two stems is not a required structural feature of the pseudoknot for promoting efficient frameshifting in SRV-1.
Conformation of a non-frameshifting RNA pseudoknot from mouse mammary tumor virus.
The solution conformation of an RNA pseudoknot, which is a mutant of the pseudoknot required for ribosomal frameshifting in mouse mammary tumor virus, has been determined by NMR. The 32-nucleotide…
A mutant RNA pseudoknot that promotes ribosomal frameshifting in mouse mammary tumor virus.
The structural similarity among the efficient frameshifting pseudoknots indicates that a specific conformation is required for ribosomal frameshifted, further implying a specific interaction of the pseudok not with the ribosome.
Direct structural evidence for formation of a stem-loop structure involved in ribosomal frameshifting in human immunodeficiency virus type 1 1 Kumho Life and Environmental Science Laboratory…
- H. Kang
- Medicine, BiologyBiochimica et Biophysica Acta (BBA) - Gene…
- 1 April 1998
Using UV melting and enzymatic mapping analyses, S1, V1, and T1 endonuclease mappings, together with UV melting analysis, clearly indicate that this sequence element of the HIV-1 mRNA frameshift site forms a stem-loop structure, not a pseudoknot structure, which supports the stem- loop structure proposed by many mutational studies.
Determination of the substrate specificity of turnip mosaic virus NIa protease using a genetic method.
To determine the substrate specificity of the NIa protease, amino acid sequences obtained from randomized sequence libraries were obtained using a screening method referred to as GASP (genetic assay for site-specific proteolysis), and a consensus substrate sequence was deduced: Yaa-Val-Arg-His-Gln decreased Ser.
Interactions of oligonucleotide analogs containing methylphosphonate internucleotide linkages and 2'-O-methylribonucleosides.
- J. M. Kean, C. Cushman, H. Kang, T. E. Leonard, P. Miller
- Biology, MedicineNucleic acids research
- 25 October 1994
Results show that the previously observed greater stability of oligo-2'-O-methylribo-12-mer/RNA duplexes versus oligodeoxyribonucleotide/RNA Duplexes extends to oligomers containing methylphosphonate linkages and that the configuration of the methylph phosphonate linkage strongly influences the stability of the duplexs.
An improved strategy for a genetic assay for site-specific proteolysis.
It is suggested that the modified strategy provides an efficient tool for the analysis of substrate sequences of a protease in vivo and false substrate sequences that are cleaved by endogenous yeast proteases can be easily recognized and eliminated from further characterization.